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Literature summary extracted from

  • Gao, Q.; Zheng, D.; Yuan, Z.
    Substrate preference of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase in Burkholderia thailandensis (2013), FEMS Microbiol. Lett., 339, 110-116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.9 expressed in Escherichia coli BL21(DE3) cells Burkholderia thailandensis
3.2.2.16 expressed in Escherichia coli BL21(DE3) cells Burkholderia thailandensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.9 0.058
-
 S-adenosyl-L-homocysteine at pH 7.5 and 25°C Burkholderia thailandensis
3.2.2.16 0.019
-
 S-methyl-5'-thioadenosine at pH 7.5 and 25°C Burkholderia thailandensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.9 34000
-
 x * 34000, SDS-PAGE Burkholderia thailandensis
3.2.2.16 34000
-
 x * 34000, SDS-PAGE Burkholderia thailandensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.16 additional information Burkholderia thailandensis the enzyme also shows weak hydrolytic activity with S-adenosylhomocysteine ?
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O Burkholderia thailandensis preferred substrate S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O Burkholderia thailandensis almost completely preferred substrate adenine + S-methyl-5-thio-D-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.9 Burkholderia thailandensis
-
-
-
3.2.2.16 Burkholderia thailandensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.16 His-bind resin column chromatography Burkholderia thailandensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.9 S-adenosyl-L-homocysteine + H2O the catalytic efficiency of the enzyme for S-adenosyl-L-homocysteine is only 0.004% of the value for S-methyl-5'-thioadenosine Burkholderia thailandensis S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.16 additional information the enzyme also shows weak hydrolytic activity with S-adenosylhomocysteine Burkholderia thailandensis ?
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O preferred substrate Burkholderia thailandensis S-methyl-5-thio-D-ribose + adenine
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O almost completely preferred substrate Burkholderia thailandensis adenine + S-methyl-5-thio-D-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.9 ? x * 34000, SDS-PAGE Burkholderia thailandensis
3.2.2.16 ? x * 34000, SDS-PAGE Burkholderia thailandensis

Synonyms

EC Number Synonyms Comment Organism
3.2.2.9 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Burkholderia thailandensis
3.2.2.9 MTAN
-
 Burkholderia thailandensis
3.2.2.16 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Burkholderia thailandensis
3.2.2.16 MTAN
-
 Burkholderia thailandensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.16 25 37
-
 Burkholderia thailandensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.9 25 37
-
 Burkholderia thailandensis
3.2.2.16 25 37
-
 Burkholderia thailandensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.9 0.00021
-
 S-adenosyl-L-homocysteine at pH 7.5 and 25°C Burkholderia thailandensis
3.2.2.16 1.8
-
 S-methyl-5'-thioadenosine at pH 7.5 and 25°C Burkholderia thailandensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.9 5 8
-
 Burkholderia thailandensis
3.2.2.16 5 8
-
 Burkholderia thailandensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.9 36
-
 S-adenosyl-L-homocysteine at pH 7.5 and 25°C Burkholderia thailandensis
3.2.2.16 95
-
 S-methyl-5'-thioadenosine at pH 7.5 and 25°C Burkholderia thailandensis