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Literature summary extracted from

  • Martínez-Espinosa, R.M.; Marhuenda-Egea, F.C.; Bonete, M.J.
    Purification and characterisation of a possible assimilatory nitrite reductase from the halophile archaeon Haloferax mediterranei (2001), FEMS Microbiol. Lett., 196, 113-118.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.7.1 the stability of the enzyme depends on the salt concentration. The maximum stability is found in the presence of 3.6 M NaCl Haloferax mediterranei
1.7.7.1 when the enzyme is dialysed against a 50 mM phosphate buffer, pH 7.0, containing 20% glycerol, the enzyme can be stored for 2 weeks without any significant loss of activity Haloferax mediterranei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 4-hydroxymercuribenzoate
-
 Haloferax mediterranei
1.7.7.1 azide
-
 Haloferax mediterranei
1.7.7.1 cyanide
-
 Haloferax mediterranei
1.7.7.1 additional information potassium chlorate and dithiothreitol are not effective inhibitors Haloferax mediterranei
1.7.7.1 sulfite
-
 Haloferax mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 1.9
-
 reduced methyl viologen pH 7.0, 60°C Haloferax mediterranei
1.7.7.1 8.6
-
 nitrite pH 7.0, 60°C Haloferax mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 66000
-
 1 * 66000 Haloferax mediterranei
1.7.7.1 74000
-
 gel filtration Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+ Haloferax mediterranei assimilatory nitrite reductase NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+ Haloferax mediterranei DSM 1411 assimilatory nitrite reductase NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Haloferax mediterranei
-
-
-
1.7.7.1 Haloferax mediterranei DSM 1411
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1
-
 Haloferax mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 15.8
-
 pH 7.0, 60°C Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 additional information no activity with NADH as electron donor Haloferax mediterranei ?
-
 ?
1.7.7.1 additional information no activity with NADH as electron donor Haloferax mediterranei DSM 1411 ?
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+ assimilatory nitrite reductase Haloferax mediterranei NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+ ferredoxin is almost as efficient as methyl viologen as electron donor Haloferax mediterranei NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+ assimilatory nitrite reductase Haloferax mediterranei DSM 1411 NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+ ferredoxin is almost as efficient as methyl viologen as electron donor Haloferax mediterranei DSM 1411 NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + FADH2 50% of maximum activity compared to reduced methyl viologen as electron donor Haloferax mediterranei NH3 + FAD + H2O
-
 ?
1.7.7.1 nitrite + FADH2 50% of maximum activity compared to reduced methyl viologen as electron donor Haloferax mediterranei DSM 1411 NH3 + FAD + H2O
-
 ?
1.7.7.1 nitrite + NADPH + H+ 10% of maximum activity compared to reduced methyl viologen as electron donor Haloferax mediterranei NH3 + NADP+ + H2O
-
 ?
1.7.7.1 nitrite + reduced 2,6-dichlorophenolindophenol less than 35% of maximum activity compared to reduced methyl viologen as electron donor Haloferax mediterranei NH3 + oxidized 2,6-dichlorophenolindophenol + H2O
-
 ?
1.7.7.1 nitrite + reduced methyl viologen ferredoxin is almost as efficient as methyl viologen as electron donor Haloferax mediterranei NH3 + H2O + oxidized methyl viologen
-
 ?
1.7.7.1 nitrite + reduced methyl viologen ferredoxin is almost as efficient as methyl viologen as electron donor Haloferax mediterranei DSM 1411 NH3 + H2O + oxidized methyl viologen
-
 ?
1.7.7.1 nitrite + reduced phenazine methosulphate less than 35% of maximum activity compared to reduced methyl viologen as electron donor Haloferax mediterranei NH3 + oxidized phenazine methosulphate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 monomer 1 * 66000 Haloferax mediterranei

Synonyms

EC Number Synonyms Comment Organism
1.7.7.1 assimilatory NiR
-
 Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.7.1 60
-
 when measured in the presence of 3.3 M NaCl Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.1 6.5
-
 at 40°C Haloferax mediterranei
1.7.7.1 7.5
-
 at 60°C Haloferax mediterranei

General Information

EC Number General Information Comment Organism
1.7.7.1 physiological function assimilatory nitrite reductase Haloferax mediterranei