EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.23 | expression in Escherichia coli with an N-terminal 6* His sequence | Hyperthermus butylicus |
2.5.1.126 | expression in Escherichia coli with an N-terminal 6His sequence | Pyrobaculum aerophilum |
2.5.1.127 | expression in Escherichia coli with an N-terminal 6* His sequence | Hyperthermus butylicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.23 | 34437 | - |
x * 34437, calculation from sequence | Hyperthermus butylicus |
2.5.1.23 | 37000 | - |
x * 37000, SDS-PAGE | Hyperthermus butylicus |
2.5.1.126 | 32800 | - |
x * 32800, calculation from sequence | Pyrobaculum aerophilum |
2.5.1.126 | 37000 | - |
x * 37000, SDS-PAGE | Pyrobaculum aerophilum |
2.5.1.127 | 32400 | - |
x * 32400, calculation from sequence | Hyperthermus butylicus |
2.5.1.127 | 35000 | - |
x * 35000, SDS-PAGE | Hyperthermus butylicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.126 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | Pyrobaculum aerophilum | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.126 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | Pyrobaculum aerophilum DSM 7523 | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | Pyrobaculum aerophilum | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | Pyrobaculum aerophilum DSM 7523 | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermidine | Pyrobaculum aerophilum | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermine | Pyrobaculum aerophilum | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldopentamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | Pyrobaculum aerophilum | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + norspermidine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + spermidine | Pyrobaculum aerophilum | spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
2.5.1.127 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | Hyperthermus butylicus | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.127 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | Hyperthermus butylicus DSM 5456 | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | Hyperthermus butylicus | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | Hyperthermus butylicus DSM 5456 | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermidine | Hyperthermus butylicus | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermidine | Hyperthermus butylicus DSM 5456 | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermine | Hyperthermus butylicus | the long-chain polyamines stabilize double-stranded DNA at high temperatures | S-methyl-5'-thioadenosine + caldopentamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + spermidine | Hyperthermus butylicus | spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.23 | Hyperthermus butylicus | A2BJU2 | - |
- |
2.5.1.23 | Hyperthermus butylicus DSM 5456 | A2BJU2 | - |
- |
2.5.1.126 | Pyrobaculum aerophilum | Q8ZXM4 | - |
- |
2.5.1.126 | Pyrobaculum aerophilum DSM 7523 | Q8ZXM4 | - |
- |
2.5.1.127 | Hyperthermus butylicus | A2BIX4 | - |
- |
2.5.1.127 | Hyperthermus butylicus DSM 5456 | A2BIX4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.23 | - |
Hyperthermus butylicus |
2.5.1.126 | - |
Pyrobaculum aerophilum |
2.5.1.127 | - |
Hyperthermus butylicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.23 | 0.002 | - |
pH 7.5, 95°C, substrate: spermidine | Hyperthermus butylicus |
2.5.1.23 | 0.038 | - |
pH 7.5, 95°C, substrate: propane-1,3-diamine | Hyperthermus butylicus |
2.5.1.126 | 0.005 | - |
pH 7.5, 95°C, substrate: norspermine | Pyrobaculum aerophilum |
2.5.1.126 | 0.052 | - |
pH 7.5, 95°C, substrate: spermidine | Pyrobaculum aerophilum |
2.5.1.126 | 0.09 | - |
pH 7.5, 95°C, substrate: propane-1,3-diamine | Pyrobaculum aerophilum |
2.5.1.126 | 0.14 | - |
pH 7.5, 95°C, substrate: norspermidine | Pyrobaculum aerophilum |
2.5.1.127 | 0.002 | - |
pH 7.5, 95°C, substrate: propane-1,3-diamine | Hyperthermus butylicus |
2.5.1.127 | 0.018 | - |
pH 7.5, 95°C, substrate: spermidine | Hyperthermus butylicus |
2.5.1.127 | 0.064 | - |
pH 7.5, 95°C, substrate: norspermidine | Hyperthermus butylicus |
2.5.1.127 | 0.112 | - |
pH 7.5, 95°C, substrate: norspermine | Hyperthermus butylicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.23 | S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | - |
Hyperthermus butylicus | S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
? | |
2.5.1.23 | S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | - |
Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
? | |
2.5.1.23 | S-adenosyl 3-(methylthio)propylamine + spermidine | at 5% of the specific activity compared to propane-1,3-diamine | Hyperthermus butylicus | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
2.5.1.23 | S-adenosyl 3-(methylthio)propylamine + spermidine | at 5% of the specific activity compared to propane-1,3-diamine | Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
2.5.1.126 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.126 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.126 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Pyrobaculum aerophilum DSM 7523 | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.126 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum DSM 7523 | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Pyrobaculum aerophilum DSM 7523 | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum DSM 7523 | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermidine | - |
Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermidine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermidine | - |
Pyrobaculum aerophilum DSM 7523 | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + caldopentamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + norspermine | at 4% of the specific activity compared to norspermidine | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + caldopentamine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + norspermidine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | at 64% of the specific activity compared to norspermidine | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + norspermidine | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + spermidine | spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
2.5.1.126 | S-adenosyl 3-(methylthio)propylamine + spermidine | at 37% of the specific activity compared to norspermidine | Pyrobaculum aerophilum | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
2.5.1.127 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Hyperthermus butylicus | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.127 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.127 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.127 | 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + caldoheptamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Hyperthermus butylicus | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | - |
Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + caldopentamine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + caldohexamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermidine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermidine | at 57% of the specific activity compared to norspermine | Hyperthermus butylicus | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermidine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + norspermine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermine | - |
Hyperthermus butylicus | S-methyl-5'-thioadenosine + caldopentamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + norspermine | the long-chain polyamines stabilize double-stranded DNA at high temperatures | Hyperthermus butylicus | S-methyl-5'-thioadenosine + caldopentamine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | at 2% of the specific activity compared to norspermine | Hyperthermus butylicus | S-methyl-5'-thioadenosine + norspermidine | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + spermidine | spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines | Hyperthermus butylicus | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
2.5.1.127 | S-adenosyl 3-(methylthio)propylamine + spermidine | at 16% of the specific activity compared to norspermine | Hyperthermus butylicus | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.23 | ? | x * 37000, SDS-PAGE | Hyperthermus butylicus |
2.5.1.23 | ? | x * 34437, calculation from sequence | Hyperthermus butylicus |
2.5.1.126 | ? | x * 37000, SDS-PAGE | Pyrobaculum aerophilum |
2.5.1.126 | ? | x * 32800, calculation from sequence | Pyrobaculum aerophilum |
2.5.1.127 | ? | x * 35000, SDS-PAGE | Hyperthermus butylicus |
2.5.1.127 | ? | x * 32400, calculation from sequence | Hyperthermus butylicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.23 | HbSpe 1 | - |
Hyperthermus butylicus |
2.5.1.126 | long-chain polyamine synthase | ambiguous | Pyrobaculum aerophilum |
2.5.1.126 | PaSpeE | - |
Pyrobaculum aerophilum |
2.5.1.127 | HbSpe II | - |
Hyperthermus butylicus |
2.5.1.127 | long-chain polyamine synthase | ambiguous | Hyperthermus butylicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.23 | 95 | - |
assay at | Hyperthermus butylicus |
2.5.1.126 | 95 | - |
assay at | Pyrobaculum aerophilum |
2.5.1.127 | 95 | - |
assay at | Hyperthermus butylicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.23 | 7.5 | - |
assay at | Hyperthermus butylicus |
2.5.1.126 | 7.5 | - |
assay at | Pyrobaculum aerophilum |
2.5.1.127 | 7.5 | - |
assay at | Hyperthermus butylicus |