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Literature summary extracted from

  • Knott, J.M.
    Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum (2009), FEBS Lett., 583, 3519-3524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.23 expression in Escherichia coli with an N-terminal 6* His sequence Hyperthermus butylicus
2.5.1.126 expression in Escherichia coli with an N-terminal 6His sequence Pyrobaculum aerophilum
2.5.1.127 expression in Escherichia coli with an N-terminal 6* His sequence Hyperthermus butylicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.23 34437
-
x * 34437, calculation from sequence Hyperthermus butylicus
2.5.1.23 37000
-
x * 37000, SDS-PAGE Hyperthermus butylicus
2.5.1.126 32800
-
x * 32800, calculation from sequence Pyrobaculum aerophilum
2.5.1.126 37000
-
x * 37000, SDS-PAGE Pyrobaculum aerophilum
2.5.1.127 32400
-
x * 32400, calculation from sequence Hyperthermus butylicus
2.5.1.127 35000
-
x * 35000, SDS-PAGE Hyperthermus butylicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.126 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.126 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum DSM 7523 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum DSM 7523 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermidine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldopentamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermidine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + spermidine Pyrobaculum aerophilum spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines S-methyl-5'-thioadenosine + thermospermine + H+
-
?
2.5.1.127 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.127 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus DSM 5456 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus DSM 5456 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermidine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermidine Hyperthermus butylicus DSM 5456 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldopentamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + spermidine Hyperthermus butylicus spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines S-methyl-5'-thioadenosine + thermospermine + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.23 Hyperthermus butylicus A2BJU2
-
-
2.5.1.23 Hyperthermus butylicus DSM 5456 A2BJU2
-
-
2.5.1.126 Pyrobaculum aerophilum Q8ZXM4
-
-
2.5.1.126 Pyrobaculum aerophilum DSM 7523 Q8ZXM4
-
-
2.5.1.127 Hyperthermus butylicus A2BIX4
-
-
2.5.1.127 Hyperthermus butylicus DSM 5456 A2BIX4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.23
-
Hyperthermus butylicus
2.5.1.126
-
Pyrobaculum aerophilum
2.5.1.127
-
Hyperthermus butylicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.23 0.002
-
pH 7.5, 95°C, substrate: spermidine Hyperthermus butylicus
2.5.1.23 0.038
-
pH 7.5, 95°C, substrate: propane-1,3-diamine Hyperthermus butylicus
2.5.1.126 0.005
-
pH 7.5, 95°C, substrate: norspermine Pyrobaculum aerophilum
2.5.1.126 0.052
-
pH 7.5, 95°C, substrate: spermidine Pyrobaculum aerophilum
2.5.1.126 0.09
-
pH 7.5, 95°C, substrate: propane-1,3-diamine Pyrobaculum aerophilum
2.5.1.126 0.14
-
pH 7.5, 95°C, substrate: norspermidine Pyrobaculum aerophilum
2.5.1.127 0.002
-
pH 7.5, 95°C, substrate: propane-1,3-diamine Hyperthermus butylicus
2.5.1.127 0.018
-
pH 7.5, 95°C, substrate: spermidine Hyperthermus butylicus
2.5.1.127 0.064
-
pH 7.5, 95°C, substrate: norspermidine Hyperthermus butylicus
2.5.1.127 0.112
-
pH 7.5, 95°C, substrate: norspermine Hyperthermus butylicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.23 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
-
Hyperthermus butylicus S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
?
2.5.1.23 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
-
Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
?
2.5.1.23 S-adenosyl 3-(methylthio)propylamine + spermidine at 5% of the specific activity compared to propane-1,3-diamine Hyperthermus butylicus S-methyl-5'-thioadenosine + thermospermine + H+
-
?
2.5.1.23 S-adenosyl 3-(methylthio)propylamine + spermidine at 5% of the specific activity compared to propane-1,3-diamine Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + thermospermine + H+
-
?
2.5.1.126 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.126 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.126 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.126 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermidine
-
Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermidine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermidine
-
Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldopentamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + norspermine at 4% of the specific activity compared to norspermidine Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldopentamine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermidine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine at 64% of the specific activity compared to norspermidine Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermidine
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + spermidine spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines Pyrobaculum aerophilum S-methyl-5'-thioadenosine + thermospermine + H+
-
?
2.5.1.126 S-adenosyl 3-(methylthio)propylamine + spermidine at 37% of the specific activity compared to norspermidine Pyrobaculum aerophilum S-methyl-5'-thioadenosine + thermospermine + H+
-
?
2.5.1.127 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Hyperthermus butylicus S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.127 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.127 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.127 2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldoheptamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Hyperthermus butylicus S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldohexamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermidine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermidine at 57% of the specific activity compared to norspermine Hyperthermus butylicus S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermidine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + norspermine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermine
-
Hyperthermus butylicus S-methyl-5'-thioadenosine + caldopentamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + norspermine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + caldopentamine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine at 2% of the specific activity compared to norspermine Hyperthermus butylicus S-methyl-5'-thioadenosine + norspermidine
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + spermidine spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines Hyperthermus butylicus S-methyl-5'-thioadenosine + thermospermine + H+
-
?
2.5.1.127 S-adenosyl 3-(methylthio)propylamine + spermidine at 16% of the specific activity compared to norspermine Hyperthermus butylicus S-methyl-5'-thioadenosine + thermospermine + H+
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.23 ? x * 37000, SDS-PAGE Hyperthermus butylicus
2.5.1.23 ? x * 34437, calculation from sequence Hyperthermus butylicus
2.5.1.126 ? x * 37000, SDS-PAGE Pyrobaculum aerophilum
2.5.1.126 ? x * 32800, calculation from sequence Pyrobaculum aerophilum
2.5.1.127 ? x * 35000, SDS-PAGE Hyperthermus butylicus
2.5.1.127 ? x * 32400, calculation from sequence Hyperthermus butylicus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.23 HbSpe 1
-
Hyperthermus butylicus
2.5.1.126 long-chain polyamine synthase ambiguous Pyrobaculum aerophilum
2.5.1.126 PaSpeE
-
Pyrobaculum aerophilum
2.5.1.127 HbSpe II
-
Hyperthermus butylicus
2.5.1.127 long-chain polyamine synthase ambiguous Hyperthermus butylicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.23 95
-
assay at Hyperthermus butylicus
2.5.1.126 95
-
assay at Pyrobaculum aerophilum
2.5.1.127 95
-
assay at Hyperthermus butylicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.23 7.5
-
assay at Hyperthermus butylicus
2.5.1.126 7.5
-
assay at Pyrobaculum aerophilum
2.5.1.127 7.5
-
assay at Hyperthermus butylicus