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Literature summary extracted from
- The influence of identity elements on the aminoacylation of tRNAArg by plant and Escherichia coli arginyl-tRNA synthetases (2012), FEBS J., 279, 3622-3638.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.1.1.19 | expressed in Escherichia coli BL21 Codon Plus RP cells | Canavalia ensiformis |
| 6.1.1.19 | expressed in Escherichia coli BL21 Codon Plus RP cells | Glycine max |
| 6.1.1.19 | expressed in Escherichia coli BL21 Codon Plus RP cells | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.19 | 0.00029 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
| 6.1.1.19 | 0.0014 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 6.1.1.19 | 0.0017 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
| 6.1.1.19 | 0.0022 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 6.1.1.19 | 0.0024 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
| 6.1.1.19 | 0.0041 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.19 | Canavalia ensiformis | B2G3G6 | - |
- |
| 6.1.1.19 | Escherichia coli | B1XHE4 | - |
- |
| 6.1.1.19 | Glycine max | B6ETP1 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.19 | Ni-NTA column chromatography and Source 15Q column chromatography | Glycine max |
| 6.1.1.19 | Ni-NTA column chromatography and Source15Q column chromatography | Canavalia ensiformis |
| 6.1.1.19 | Ni-NTA column chromatography and Source15Q column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.19 | ATP + L-arginine + tRNAArg(ACG) | tRNA from Escherichia coli | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg(ACG) | - |
? |  |
| 6.1.1.19 | ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg(ACG) | - |
? | |
| 6.1.1.19 | ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Escherichia coli | AMP + diphosphate + L-arginyl-soybean tRNAArg(ACG) | - |
? | |
| 6.1.1.19 | ATP + L-arginine + tRNAArg(ACG) | tRNA from Escherichia coli | Glycine max | AMP + diphosphate + L-arginyl-tRNAArg(ACG) | - |
? | |
| 6.1.1.19 | ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Glycine max | AMP + diphosphate + L-arginyl-tRNAArg(ACG) | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.1.1.19 | Arginyl-tRNA synthetase | - |
Canavalia ensiformis |
| 6.1.1.19 | Arginyl-tRNA synthetase | - |
Glycine max |
| 6.1.1.19 | Arginyl-tRNA synthetase | - |
Escherichia coli |
| 6.1.1.19 | ArgRS | - |
Canavalia ensiformis |
| 6.1.1.19 | ArgRS | - |
Glycine max |
| 6.1.1.19 | ArgRS | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.19 | 0.0017 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
| 6.1.1.19 | 0.02 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli | |
| 6.1.1.19 | 0.03 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
| 6.1.1.19 | 0.04 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
| 6.1.1.19 | 0.055 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 6.1.1.19 | 0.105 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.1.1.19 | ATP | - |
Canavalia ensiformis | |
| 6.1.1.19 | ATP | - |
Glycine max | |
| 6.1.1.19 | ATP | - |
Escherichia coli | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.19 | 4.8 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli | |
| 6.1.1.19 | 5.5 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
| 6.1.1.19 | 14 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
| 6.1.1.19 | 22.7 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
| 6.1.1.19 | 25.2 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 6.1.1.19 | 75 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis |
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