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Literature summary extracted from

  • Ronconi, S.; Jonczyk, R.; Genschel, U.
    A novel isoform of pantothenate synthetase in the Archaea (2008), FEBS J., 275, 2754-2764.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.44 expression in Escherichia coli as a N-terminally His-tag fusion protein Methanosarcina mazei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.44 (R)-pantothenate strong product inhibition Methanosarcina mazei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.44 30000
-
 2 * 30000, SDS-PAGE Methanosarcina mazei
6.3.2.44 57000
-
 gel filtration Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.44 ATP + (R)-pantoate + beta-alanine Methanosarcina mazei the enzyme is involved in the biosynthesis of pantothenate ADP + phosphate + (R)-pantothenate
-
 ?
6.3.2.44 ATP + (R)-pantoate + beta-alanine Methanosarcina mazei DSM 3647 the enzyme is involved in the biosynthesis of pantothenate ADP + phosphate + (R)-pantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.44 Methanosarcina mazei Q8PUQ1
-
-
6.3.2.44 Methanosarcina mazei DSM 3647 Q8PUQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.44
-
 Methanosarcina mazei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.44 0.14
-
 pH 8.0, 37°C Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.44 ATP + (R)-pantoate + beta-alanine the enzyme is involved in the biosynthesis of pantothenate Methanosarcina mazei ADP + phosphate + (R)-pantothenate
-
 ?
6.3.2.44 ATP + (R)-pantoate + beta-alanine purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase Methanosarcina mazei ADP + phosphate + (R)-pantothenate
-
 ?
6.3.2.44 ATP + (R)-pantoate + beta-alanine the enzyme is involved in the biosynthesis of pantothenate Methanosarcina mazei DSM 3647 ADP + phosphate + (R)-pantothenate
-
 ?
6.3.2.44 ATP + (R)-pantoate + beta-alanine purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase Methanosarcina mazei DSM 3647 ADP + phosphate + (R)-pantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.44 dimer 2 * 30000, SDS-PAGE Methanosarcina mazei

Synonyms

EC Number Synonyms Comment Organism
6.3.2.44 MM2281 locus name Methanosarcina mazei
6.3.2.44 Pantothenate synthetase ambiguous Methanosarcina mazei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.44 37
-
 assay at Methanosarcina mazei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.44 8
-
 assay at Methanosarcina mazei

General Information

EC Number General Information Comment Organism
6.3.2.44 physiological function the enzyme is involved in the biosynthesis of pantothenate Methanosarcina mazei