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Literature summary extracted from

  • Pire, C.; Martínez-Espinosa, R.M.; Perez-Pomares, F.; Esclapez, J.; Bonete, M.J.
    Ferredoxin-dependent glutamate synthase: involvement in ammonium assimilation in Haloferax mediterranei (2013), Extremophiles, 18, 147-159.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.7.1 0.018
-
 reduced ferredoxin pH 7.5, 40°C, 3 M NaCl Haloferax mediterranei
1.4.7.1 2.1
-
 L-glutamine pH 7.5, 40°C, 3 M NaCl Haloferax mediterranei
1.4.7.1 31
-
 2-oxoglutarate pH 7.5, 40°C, 3 M NaCl Haloferax mediterranei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.7.1 NaCl maximal activity at salt concentrations from 3 to 4 M NaCl Haloferax mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.7.1 163780
-
 1 * 163780, calculated from sequence Haloferax mediterranei
1.4.7.1 198000
-
 gel filtration Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.7.1 2 L-glutamate + 2 oxidized ferredoxin Haloferax mediterranei overall reaction. The enzyme is involved in the ammonium assimilation pathway which enables Haloferax mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+
-
 ?
1.4.7.1 2 L-glutamate + 2 oxidized ferredoxin Haloferax mediterranei ATCC 33500 overall reaction. The enzyme is involved in the ammonium assimilation pathway which enables Haloferax mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.7.1 Haloferax mediterranei C0MP41
-
-
1.4.7.1 Haloferax mediterranei ATCC 33500 C0MP41
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.7.1 2 L-glutamate + 2 oxidized ferredoxin overall reaction. The enzyme is involved in the ammonium assimilation pathway which enables Haloferax mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source Haloferax mediterranei L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+
-
 ?
1.4.7.1 2 L-glutamate + 2 oxidized ferredoxin overall reaction. The enzyme is involved in the ammonium assimilation pathway which enables Haloferax mediterranei to thrive in media with low ammonium concentration or containing just nitrate as single nitrogen source Haloferax mediterranei ATCC 33500 L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ no activity is detected in presence of either 5 mM NADH or 0.5 mM NADPH. Methyl viologen at 20 mM and 0.004 mM ferredoxin I are also tested under the same conditions: activity with 0.004 mM ferredoxin I as reducing agent is approximately 3fold higher than that measured with 20 mM methyl viologen. For the other ferrodoxin isoform found in Haloferax mediterranei, ferrodoxin II, the activity is approximately 8fold lower than that for ferredoxin I Haloferax mediterranei 2 L-glutamate + 2 oxidized ferredoxin
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ no activity is detected in presence of either 5 mM NADH or 0.5 mM NADPH. Methyl viologen at 20 mM and 0.004 mM ferredoxin I are also tested under the same conditions: activity with 0.004 mM ferredoxin I as reducing agent is approximately 3fold higher than that measured with 20 mM methyl viologen. For the other ferrodoxin isoform found in Haloferax mediterranei, ferrodoxin II, the activity is approximately 8fold lower than that for ferredoxin I Haloferax mediterranei ATCC 33500 2 L-glutamate + 2 oxidized ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.7.1 monomer 1 * 163780, calculated from sequence Haloferax mediterranei

Synonyms

EC Number Synonyms Comment Organism
1.4.7.1 ferredoxin-dependent glutamate synthase
-
 Haloferax mediterranei
1.4.7.1 GltS
-
 Haloferax mediterranei
1.4.7.1 GOGAT
-
 Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.7.1 50
-
-
 Haloferax mediterranei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.7.1 30 80 30°C: about 75% of maximal activity, 80°C: about 55% of maximal activity Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.7.1 7.5
-
-
 Haloferax mediterranei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.7.1 5 10 pH 5.0: about 45% of maximal activity, pH 10.0: about 55% of maximal activity Haloferax mediterranei

Expression

EC Number Organism Comment Expression
1.4.7.1 Haloferax mediterranei expression is induced under conditions of ammonium restriction up