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Literature summary extracted from

  • Ishibashi, T.; Tomita, H.; Yokooji, Y.; Morikita, T.; Watanabe, B.; Hiratake, J.; Kishimoto, A.; Kita, A.; Miki, K.; Imanaka, T.; Atomi, H.
    A detailed biochemical characterization of phosphopantothenate synthetase, a novel enzyme involved in coenzyme A biosynthesis in the Archaea (2012), Extremophiles, 16, 819-828.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 the TK2141 gene encoding PoK, with a His6-Tag on its N-terminus, is overexpressed in Thermococcus kodakarensis Thermococcus kodakarensis
6.3.2.26 overexpressed in Escherichoa coli Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 4-phosphopantoate substrate inhibition Thermococcus kodakarensis
2.7.1.33 ATP substrate inhibition Thermococcus kodakarensis
2.7.1.33 additional information feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed Thermococcus kodakarensis
6.3.2.26 4-phosphopantoate substrate inhibition Thermococcus kodakarensis
6.3.2.26 ATP substrate inhibition Thermococcus kodakarensis
6.3.2.26 additional information feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.26 0.34
-
 beta-Alanine pH 6.5, 85°C Thermococcus kodakarensis
6.3.2.26 1.16
-
 (R)-4-phosphopantoate pH 6.5, 85°C Thermococcus kodakarensis
6.3.2.26 2.44
-
 ATP pH 6.5, 85°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-4-phosphopantoate + beta-alanine Thermococcus kodakarensis the enzyme is involved in coenzyme A biosynthesis in the archaea AMP + diphosphate + (R)-4'-phosphopantothenate
-
 ?
6.3.2.26 ATP + (R)-4-phosphopantoate + beta-alanine Thermococcus kodakarensis the enzyme is involved in coenzyme A biosynthesis in the archaea AMP + diphosphate + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Thermococcus kodakarensis Q5JHF1
-
-
6.3.2.26 Thermococcus kodakarensis Q5JIZ8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33
-
 Thermococcus kodakarensis
6.3.2.26
-
 Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.26 6.38
-
 beta-alanine, pH 6.5, 85°C Thermococcus kodakarensis
6.3.2.26 14.7
-
 ATP, pH 6.5, 85°C Thermococcus kodakarensis
6.3.2.26 15.6
-
 (R)-4-phosphopantoate, pH 6.5, 85°C Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-4-phosphopantoate + beta-alanine the enzyme is involved in coenzyme A biosynthesis in the archaea Thermococcus kodakarensis AMP + diphosphate + (R)-4'-phosphopantothenate
-
 ?
6.3.2.26 ATP + (R)-4-phosphopantoate + beta-alanine the enzyme is involved in coenzyme A biosynthesis in the archaea Thermococcus kodakarensis AMP + diphosphate + (R)-4'-phosphopantothenate
-
 ?
6.3.2.26 ATP + (R)-4-phosphopantoate + beta-alanine strict preference for ATP. Among several amine substrates, activity is detected with beta-alanine, but not with gamma-aminobutyrate, glycine nor aspartate Thermococcus kodakarensis AMP + diphosphate + (R)-4'-phosphopantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 4-phosphopantoate non-competitive Thermococcus kodakarensis
2.7.1.33 PoK
-
 Thermococcus kodakarensis
2.7.1.33 TK2141
-
 Thermococcus kodakarensis
6.3.2.26 phosphopantothenate synthetase
-
 Thermococcus kodakarensis
6.3.2.26 Pps
-
 Thermococcus kodakarensis
6.3.2.26 TK1686
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.33 75
-
 assay at Thermococcus kodakarensis
6.3.2.26 85
-
 assay at Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.26 65 95 activity continues to increase with temperature elevation from 65°C up to 95°C Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.26 70
-
 6 h, no decrease in activity Thermococcus kodakarensis
6.3.2.26 80
-
 half-life: 13.6 h Thermococcus kodakarensis
6.3.2.26 90
-
 half-life: 8.2 h Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 8
-
 assay at Thermococcus kodakarensis
6.3.2.26 6.5
-
-
 Thermococcus kodakarensis