Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, S.M.; Paek, K.H.; Lee, S.B.
    Characterization of NADP+-specific L-rhamnose dehydrogenase from the thermoacidophilic Archaeon Thermoplasma acidophilum. (2012), Extremophiles, 16, 447-454.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.377 expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.377 0.1
-
NADP+ pH 7.0, 60°C, cosubstrate: L-lyxose Thermoplasma acidophilum
1.1.1.377 0.1
-
NADP+ pH 7.0, 60°C, cosubstrate: L-rhamnose Thermoplasma acidophilum
1.1.1.377 0.13
-
NADP+ pH 7.0, 60°C, cosubstrate: L-mannose Thermoplasma acidophilum
1.1.1.377 0.46
-
L-rhamnose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 1.37
-
L-Lyxose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 6.74
-
L-Mannose pH 7.0, 60°C Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.377 31237
-
x * 31237, calculated from sequence4 Thermoplasma acidophilum
1.1.1.377 34000
-
x * 34000, SDS-PAGE Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.377 L-rhamnose + NADP+ Thermoplasma acidophilum first enzyme of the non-phosphorylative L-rhamnose pathway in archaea L-rhamno-1,4-lactone + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.377 Thermoplasma acidophilum G3LGQ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.377
-
Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.377 16.2
-
pH 7.0, 60°C, substrates: L-mannose + NADP+ Thermoplasma acidophilum
1.1.1.377 35.1
-
pH 7.0, 60°C, substrates: L-lyxose + NADP+ Thermoplasma acidophilum
1.1.1.377 36.2
-
pH 7.0, 60°C, substrates: L-rhamnose + NADP+ Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.377 L-lyxose + NADP+
-
Thermoplasma acidophilum ?
-
?
1.1.1.377 L-mannose + NADP+
-
Thermoplasma acidophilum ?
-
?
1.1.1.377 L-rhamnose + NADP+
-
Thermoplasma acidophilum L-rhamno-1,4-lactone + NADPH + H+
-
?
1.1.1.377 L-rhamnose + NADP+ first enzyme of the non-phosphorylative L-rhamnose pathway in archaea Thermoplasma acidophilum L-rhamno-1,4-lactone + NADPH + H+
-
?
1.1.1.377 additional information less than 1% activity is observed for D-mannose, myo-inositol, D-glyceraldehyde, and D-talose. No catalytic activity is detected for the following substrates: D-glucose, D-galactose, L-galactose, D-arabinose, L-arabinose, Dxylose, L-xylose, D-fucose, L-fucose, D-fructose, D-mannitol, D-xylitol, D-sorbitol, D-ribose, D-deoxyribose, D-glucosamine, N-acetyl-D-glucosamine, and D-altrose Thermoplasma acidophilum ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.377 ? x * 34000, SDS-PAGE Thermoplasma acidophilum
1.1.1.377 ? x * 31237, calculated from sequence4 Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.377 NADP+-specific L-rhamnose dehydrogenase
-
Thermoplasma acidophilum
1.1.1.377 RhaD
-
Thermoplasma acidophilum
1.1.1.377 Ta0747P locus name Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.377 60
-
activity towards L-rhamnose Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.377 14.88
-
L-Mannose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 16.5
-
NADP+ pH 7.0, 60°C, cosubstrate: L-lyxose Thermoplasma acidophilum
1.1.1.377 17.1
-
NADP+ pH 7.0, 60°C, cosubstrate: L-rhamnose Thermoplasma acidophilum
1.1.1.377 18.08
-
NADP+ pH 7.0, 60°C, cosubstrate: L-mannose Thermoplasma acidophilum
1.1.1.377 22.36
-
L-rhamnose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 23.77
-
L-Lyxose pH 7.0, 60°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.377 7
-
activity towards L-rhamnose Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.377 NADP+ no activity with NAD+ Thermoplasma acidophilum

General Information

EC Number General Information Comment Organism
1.1.1.377 physiological function first enzyme of the non-phosphorylative L-rhamnose pathway in archaea Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.377 2.2
-
L-Mannose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 17.35
-
L-Lyxose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 48.6
-
L-rhamnose pH 7.0, 60°C Thermoplasma acidophilum
1.1.1.377 139
-
NADP+ pH 7.0, 60°C, cosubstrate: L-mannose Thermoplasma acidophilum
1.1.1.377 165
-
NADP+ pH 7.0, 60°C, cosubstrate: L-lyxose Thermoplasma acidophilum
1.1.1.377 171
-
NADP+ pH 7.0, 60°C, cosubstrate: L-rhamnose Thermoplasma acidophilum