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Literature summary extracted from

  • Richter, O.M.; Schäfer, G.
    Purification and enzymic characterization of the cytoplasmic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum (1992), Eur. J. Biochem., 209, 343-349.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 Ca2+ inhibits the cleavage of diphosphate in the presence of 2.5 mM Mg2+. 0.01 mM CaCl2 inhibits the enzyme by about 50% Thermoplasma acidophilum
3.6.1.1 Phenylglyoxal the inhibitory effect of phenylglyoxal is higher at 56°C than at room temperature. With 5 mM phenylglyoxal almost complete inhibition can be observed after 15 min incubation at 56°C Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.007
-
 diphosphate pH 6.2, 56°C Thermoplasma acidophilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 cytoplasm the enzyme occurs almost exclusively in the cytoplasmic fraction Thermoplasma acidophilum 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ absolute requirement for divalent cations, 2.5 mM Mn2+ activates 37% compared to 2.5 mM Mg2+ Thermoplasma acidophilum
3.6.1.1 Mg2+ absolute requirement for divalent cations for catalytic action, Mg2 + (2.5 mM) conferring the highest activity. KM for Mg2+ is approximately 1.7 mM Thermoplasma acidophilum
3.6.1.1 Mn2+ absolute requirement for divalent cations, 2.5 mM Mn2+ activates 17% compared to 2.5 mM Mg2+ Thermoplasma acidophilum
3.6.1.1 Zn2+ absolute requirement for divalent cations, 2.5 mM Mn2+ activates 21% compared to 2.5 mM Mg2+ Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 22000
-
 6 * 22000, SDS-PAGE Thermoplasma acidophilum
3.6.1.1 110000
-
 gel filtration Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Thermoplasma acidophilum P37981
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 1200
-
 pH 6.2, 56°C Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O pronounced specificity for diphosphate Thermoplasma acidophilum 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 hexamer 6 * 22000, SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 85
-
-
 Thermoplasma acidophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.1 70 100 70°C: about 70% of maximal activity, 100°C: about 80% of maximal activity Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 2200
-
 diphosphate pH 6.2, 56°C Thermoplasma acidophilum