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Literature summary extracted from

  • Fiebig, K.; Friedrich, B.
    Purification of the F420-reducing hydrogenase from Methanosarcina barkeri (strain Fusaro) (1989), Eur. J. Biochem., 184, 79-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.98.1 additional information conditions for anaerobic reductive activation in the presence of hydrogen, 2-mercaptoethanol and KCl or methyl viologen yield maximal hydrogenase activity Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.1 0.0033
-
 oxidized methyl viologen 37°C, pH 8.3 Methanosarcina barkeri
1.12.98.1 0.025
-
 oxidized coenzyme F420 37°C, pH 7.5 Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 KCl increases activity, maximal activity at 250 mM Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 8000
-
 x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri
1.12.98.1 30000
-
 x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri
1.12.98.1 33000
-
 x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri
1.12.98.1 198000
-
 non-denaturing PAGE, small enzyme form Methanosarcina barkeri
1.12.98.1 845000
-
 non-denaturing PAGE, large enzyme form Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + oxidized coenzyme F420 Methanosarcina barkeri
-
 reduced coenzyme F420
-
 ?
1.12.98.1 H2 + oxidized coenzyme F420 Methanosarcina barkeri DSM 804
-
 reduced coenzyme F420
-
 ?
1.12.98.1 H2 + oxidized methyl viologen Methanosarcina barkeri
-
 reduced methyl viologen
-
 ?
1.12.98.1 H2 + oxidized methyl viologen Methanosarcina barkeri DSM 804
-
 reduced methyl viologen
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanosarcina barkeri P80490 and P80489 and P80491 P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma
-
1.12.98.1 Methanosarcina barkeri DSM 804 P80490 and P80489 and P80491 P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.1 enzyme activity is unstable under reducing conditions Methanosarcina barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 11.5
-
 37°C, pH 7.5, substrate: oxidized coenzyme F420 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + oxidized coenzyme F420
-
 Methanosarcina barkeri reduced coenzyme F420
-
 ?
1.12.98.1 H2 + oxidized coenzyme F420
-
 Methanosarcina barkeri DSM 804 reduced coenzyme F420
-
 ?
1.12.98.1 H2 + oxidized methyl viologen
-
 Methanosarcina barkeri reduced methyl viologen
-
 ?
1.12.98.1 H2 + oxidized methyl viologen
-
 Methanosarcina barkeri DSM 804 reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 ? x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
1.12.98.1 F420-reducing hydrogenase
-
 Methanosarcina barkeri
1.12.98.1 Mbar_A0449 subunit beta Methanosarcina barkeri
1.12.98.1 Mbar_A0450 subunit gamma Methanosarcina barkeri
1.12.98.1 Mbar_A0452 subunit alpha Methanosarcina barkeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.98.1 353
-
 oxidized coenzyme F420 37°C, pH 7.5 Methanosarcina barkeri
1.12.98.1 9226
-
 oxidized methyl viologen 37°C, pH 8.3 Methanosarcina barkeri