EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.10 | - |
Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.2.10 | additional information | design of a surface-modified variant incorporating 11 changes in the amino acids on the protein surface to stabilze the protein at acidic pH. Mutant shows a broadened pH optimum towards the acidic range, along with enhancement of activity by up to twofold and significantly increased pH- and thermostabilities. The effect is probably due to a shift of the isoelectic point from pH 5.1 to 4.8. Mutant is applicable in aqueous/organic two-phase systems | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.10 | Arabidopsis thaliana | Q9LFT6 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 12.2 | - |
wild-type, 25°C, pH 4.75 | Arabidopsis thaliana |
4.1.2.10 | 23.6 | - |
surface-modified mutant, 25°C, pH 4.5 | Arabidopsis thaliana |
4.1.2.10 | 133.6 | - |
wild-type, 25°C, pH 6.0 | Arabidopsis thaliana |
4.1.2.10 | 227 | - |
surface-modified mutant, 25°C, pH 5.75 | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.10 | (R)-mandelonitrile | - |
Arabidopsis thaliana | cyanide + benzaldehyde | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | - |
- |
pH 5.0, wild-type, half-life 18 min, surface-modified mutant, half-life 137 min | Arabidopsis thaliana |
4.1.2.10 | 10 | - |
pH 5.0, wild-type, half-life 23.6 min, surface-modified mutant, half-life 136 min | Arabidopsis thaliana |
4.1.2.10 | 20 | - |
pH 5.0, wild-type, half-life 9.6 min, surface-modified mutant, half-life 134 min | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 5.75 | - |
surface-modified mutant | Arabidopsis thaliana |
4.1.2.10 | 6 | - |
wild-type | Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 4.5 | - |
surface-modified mutant, less than 10% of maximum activity | Arabidopsis thaliana |
4.1.2.10 | 4.75 | - |
wild-type, less than 10% of maximum activity | Arabidopsis thaliana |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 5 | - |
20°C, wild-type, half-life 0.16 h, surface-modified mutant, half-life 2.23 h | Arabidopsis thaliana |
4.1.2.10 | 6 | - |
20°C, wild-type, half-life 60 h, surface-modified mutant, half-life 52.2 h | Arabidopsis thaliana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.1.2.10 | Arabidopsis thaliana | isoelectric focusing, wild-type | - |
5.1 |