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Literature summary extracted from

  • Okrob, D.; Metzner, J.; Wiechert, W.; Gruber, K.; Pohl, M.
    Tailoring a stabilized variant of hydroxynitrile lyase from Arabidopsis thaliana (2012), ChemBioChem, 13, 797-802.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10
-
 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.10 additional information design of a surface-modified variant incorporating 11 changes in the amino acids on the protein surface to stabilze the protein at acidic pH. Mutant shows a broadened pH optimum towards the acidic range, along with enhancement of activity by up to twofold and significantly increased pH- and thermostabilities. The effect is probably due to a shift of the isoelectic point from pH 5.1 to 4.8. Mutant is applicable in aqueous/organic two-phase systems Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Arabidopsis thaliana Q9LFT6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.10 12.2
-
 wild-type, 25°C, pH 4.75 Arabidopsis thaliana
4.1.2.10 23.6
-
 surface-modified mutant, 25°C, pH 4.5 Arabidopsis thaliana
4.1.2.10 133.6
-
 wild-type, 25°C, pH 6.0 Arabidopsis thaliana
4.1.2.10 227
-
 surface-modified mutant, 25°C, pH 5.75 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Arabidopsis thaliana cyanide + benzaldehyde
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10
-
-
 pH 5.0, wild-type, half-life 18 min, surface-modified mutant, half-life 137 min Arabidopsis thaliana
4.1.2.10 10
-
 pH 5.0, wild-type, half-life 23.6 min, surface-modified mutant, half-life 136 min Arabidopsis thaliana
4.1.2.10 20
-
 pH 5.0, wild-type, half-life 9.6 min, surface-modified mutant, half-life 134 min Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 5.75
-
 surface-modified mutant Arabidopsis thaliana
4.1.2.10 6
-
 wild-type Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.10 4.5
-
 surface-modified mutant, less than 10% of maximum activity Arabidopsis thaliana
4.1.2.10 4.75
-
 wild-type, less than 10% of maximum activity Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 5
-
 20°C, wild-type, half-life 0.16 h, surface-modified mutant, half-life 2.23 h Arabidopsis thaliana
4.1.2.10 6
-
 20°C, wild-type, half-life 60 h, surface-modified mutant, half-life 52.2 h Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.10 Arabidopsis thaliana isoelectric focusing, wild-type
-
 5.1