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Literature summary extracted from

  • Jebors, S.; Tauran, Y.; Aghajari, N.; Boudebbouze, S.; Maguin, E.; Haser, R.; Coleman, A.W.; Rhimi, M.
    Supramolecular stabilization of acid tolerant L-arabinose isomerase from Lactobacillus sakei (2011), Chem. Commun. (Camb. ), 47, 12307-12309.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expressed in Escherichia coli Latilactobacillus sakei

General Stability

EC Number General Stability Organism
5.3.1.4 in the presence of 1 mM of the supramolecule NoriaPG, the enzyme preserves more than 90% of its standard activity at 40, 45 and 50°C after incubation for 2 h. Addition of 1 mM of the supramolecules Noria or NoriaPG in the presence of 0.8 mM Mg2+ and 0.8 mM Mn2+ leads to increased stabilization of the enzyme Latilactobacillus sakei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Mg2+ the enzyme activity is increased nearly 1.8fold after addition of 0.8 mM Mg2+ Latilactobacillus sakei
5.3.1.4 Mn2+ the enzyme activity is increased nearly 1.8fold after addition of 0.8 mM Mn2+ Latilactobacillus sakei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Latilactobacillus sakei
-
L-ribulose
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Latilactobacillus sakei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4
-
Latilactobacillus sakei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 L-arabinose
-
Latilactobacillus sakei L-ribulose
-
?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 D-galactose isomerase
-
Latilactobacillus sakei
5.3.1.4 L-AI
-
Latilactobacillus sakei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 30 40
-
Latilactobacillus sakei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 35 50 the enzyme is fully stable at temperatures until 35°C after 24 h. After 2 h at 40 and 45°C the enzyme maintains 95% and 60% of its initial activity, respectively, and at 50°C the half-life is 80 min. At higher temperatures the enzyme is rapidly inactivated Latilactobacillus sakei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 5 7
-
Latilactobacillus sakei