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Literature summary extracted from

  • Kato, O.; Youn, J.; Stansen, K.; Matsui, D.; Oikawa, T.; Wendisch, V.
    Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate (2010), BMC Microbiol., 10, 321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.10 expression in Escherichia coli Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.12 0.61
-
 D-lactate pH 7.5, 30°C Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.10 Corynebacterium glutamicum Q8NRY8
-
-
1.1.5.10 Corynebacterium glutamicum DSM 20300 Q8NRY8
-
-
1.1.5.12 Corynebacterium glutamicum
-
-
-
1.1.5.12 Corynebacterium glutamicum ATCC 13032
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.12 D-lactate + 2,6-dichloroindophenol
-
 Corynebacterium glutamicum pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 D-lactate + 2,6-dichloroindophenol
-
 Corynebacterium glutamicum ATCC 13032 pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 DL-2-hydroxybutanoate + 2,6-dichloroindophenol about 5% of the activity with D-lactate Corynebacterium glutamicum 2-oxobutanoate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 DL-2-hydroxybutanoate + 2,6-dichloroindophenol about 5% of the activity with D-lactate Corynebacterium glutamicum ATCC 13032 2-oxobutanoate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 L-lactate + 2,6-dichloroindophenol about 5% of the activity with D-lactate Corynebacterium glutamicum pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 L-lactate + 2,6-dichloroindophenol about 5% of the activity with D-lactate Corynebacterium glutamicum ATCC 13032 pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 additional information no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate Corynebacterium glutamicum ?
-
 ?
1.1.5.12 additional information no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate Corynebacterium glutamicum ATCC 13032 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.10 DLD
-
 Corynebacterium glutamicum
1.1.5.12 Cg1027
-
 Corynebacterium glutamicum
1.1.5.12 quinone-dependent D-lactate dehydrogenase
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.10 FAD bound cofactor Corynebacterium glutamicum
1.1.5.10 quinone
-
 Corynebacterium glutamicum
1.1.5.12 FAD
-
 Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
1.1.5.10 physiological function inactivation results in loss of the ability to grow with D-lactate. Heterologous expression in Corynebacterium efficiens enables this species to grow with D-lactate as sole carbon source Corynebacterium glutamicum
1.1.5.12 physiological function inactivation of Dld results in the loss of the ability to grow with D-lactate. Heterologous expression in Corynebacterium efficiens enables this species to grow with D-lactate as sole carbon source Corynebacterium glutamicum