Literature summary extracted from

Yildirim, D.; Tuekel, S.S.; Alagoez, D.
Crosslinked enzyme aggregates of hydroxynitrile lyase partially purified from Prunus dulcis seeds and its application for the synthesis of enantiopure cyanohydrins (2014), Biotechnol. Prog., 30, 818-827.

Application

EC Number	Application	Comment	Organism
4.1.2.10	synthesis	purification of enzyme as crosslinked enzyme aggregates and application for synthesis of enantiopure cyanohydrins	Prunus dulcis

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.2.10	Prunus dulcis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.2.10	partly, as crosslinked enzyme aggregates. Optimal conditions are initial glutaraldehyde concentration of 25% w/v, ammonium sulfate saturation concentration of 43% w/v, and crosslinking time of 18 h	Prunus dulcis

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
4.1.2.10	seed	-	Prunus dulcis	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.2.10	2-chlorobenzaldehyde + HCN	-	Prunus dulcis	(R)-2-chloromandelonitrile	after 96 h, 100% yield, 21% enantiomeric excess	?
4.1.2.10	3,4-dihydroxybenzaldehyde + HCN	-	Prunus dulcis	(R)-3,4-dihydroxymandelonitrile	after 96 h, 100% yield, 99% enantiomeric excess	?
4.1.2.10	3-phenylpropionaldehyde + HCN	-	Prunus dulcis	(R)-2-hydroxy-4-phenylbutyronitrile	after 96 h, 83% yield, 91% enantiomeric excess	?
4.1.2.10	4-bromobenzaldehyde + HCN	-	Prunus dulcis	(R)-4-bromomandelonitrile	after 96 h, 100% yield, 99% enantiomeric excess	?
4.1.2.10	4-fluorobenzaldehyde + HCN	-	Prunus dulcis	(R)-4-fluoromandelonitrile	after 96 h, 100% yield, 72% enantiomeric excess	?
4.1.2.10	4-nitrobenzaldehyde + HCN	-	Prunus dulcis	(R)-4-nitromandelonitrile	after 96 h, 100% yield, 14% enantiomeric excess	?
4.1.2.10	benzaldehyde + HCN	-	Prunus dulcis	(R)-mandelonitrile	after 96 h, 100% yield, 99% enantiomeric excess	?

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Release 2023.1 (January 2023)