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Literature summary extracted from

  • Su, J.; Wang, T.; Ma, C.; Li, Z.; Li, Z.; Wang, R.
    Homology modeling and function of trehalose synthase from Pseudomonas putida P06 (2014), Biotechnol. Lett., 36, 1009-1013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 gene treS, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.16 D112A site-directed mutagenesis, inactive mutant Pseudomonas putida
5.4.99.16 D294A site-directed mutagenesis, inactive mutant Pseudomonas putida
5.4.99.16 D403A site-directed mutagenesis, inactive mutant Pseudomonas putida
5.4.99.16 E338A site-directed mutagenesis, inactive mutant Pseudomonas putida
5.4.99.16 F115A site-directed mutagenesis, inactive mutant Pseudomonas putida
5.4.99.16 F223A site-directed mutagenesis, the mutation only modestly affects the enzymatic activity Pseudomonas putida
5.4.99.16 F255A site-directed mutagenesis, inactive mutant Pseudomonas putida
5.4.99.16 Q259A site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type enzyme Pseudomonas putida
5.4.99.16 R535A site-directed mutagenesis, the mutation only modestly affects the enzymatic activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.16 75000
-
x * 75000 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.16 maltose Pseudomonas putida
-
alpha,alpha-trehalose
-
r
5.4.99.16 maltose Pseudomonas putida P06
-
alpha,alpha-trehalose
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Pseudomonas putida
-
gene treS
-
5.4.99.16 Pseudomonas putida P06
-
gene treS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.16 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 maltose
-
Pseudomonas putida alpha,alpha-trehalose
-
r
5.4.99.16 maltose
-
Pseudomonas putida P06 alpha,alpha-trehalose
-
r
5.4.99.16 additional information TreS interconverts maltose and trehalose by an intramolecular mechanism, and therefore does not require external glucose or any other factors during the enzymatic reaction Pseudomonas putida ?
-
?
5.4.99.16 additional information TreS interconverts maltose and trehalose by an intramolecular mechanism, and therefore does not require external glucose or any other factors during the enzymatic reaction Pseudomonas putida P06 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.16 ? x * 75000 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.4.99.16 Trehalose synthase
-
Pseudomonas putida
5.4.99.16 TreS
-
Pseudomonas putida

General Information

EC Number General Information Comment Organism
5.4.99.16 additional information the highly conserved Asp294 residue is critical for catalytic activity, homology modeling and flexible docking studies of the enzyme-substrate system, overview Pseudomonas putida