EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.16 | gene treS, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.16 | D112A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
5.4.99.16 | D294A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
5.4.99.16 | D403A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
5.4.99.16 | E338A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
5.4.99.16 | F115A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
5.4.99.16 | F223A | site-directed mutagenesis, the mutation only modestly affects the enzymatic activity | Pseudomonas putida |
5.4.99.16 | F255A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
5.4.99.16 | Q259A | site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type enzyme | Pseudomonas putida |
5.4.99.16 | R535A | site-directed mutagenesis, the mutation only modestly affects the enzymatic activity | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 75000 | - |
x * 75000 | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | Pseudomonas putida | - |
alpha,alpha-trehalose | - |
r | |
5.4.99.16 | maltose | Pseudomonas putida P06 | - |
alpha,alpha-trehalose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.16 | Pseudomonas putida | - |
gene treS | - |
5.4.99.16 | Pseudomonas putida P06 | - |
gene treS | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.16 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | - |
Pseudomonas putida | alpha,alpha-trehalose | - |
r | |
5.4.99.16 | maltose | - |
Pseudomonas putida P06 | alpha,alpha-trehalose | - |
r | |
5.4.99.16 | additional information | TreS interconverts maltose and trehalose by an intramolecular mechanism, and therefore does not require external glucose or any other factors during the enzymatic reaction | Pseudomonas putida | ? | - |
? | |
5.4.99.16 | additional information | TreS interconverts maltose and trehalose by an intramolecular mechanism, and therefore does not require external glucose or any other factors during the enzymatic reaction | Pseudomonas putida P06 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.16 | ? | x * 75000 | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.16 | Trehalose synthase | - |
Pseudomonas putida |
5.4.99.16 | TreS | - |
Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.16 | additional information | the highly conserved Asp294 residue is critical for catalytic activity, homology modeling and flexible docking studies of the enzyme-substrate system, overview | Pseudomonas putida |