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Literature summary extracted from
- Oxidative decarboxylation of mandelic acid derivative by recombinant Escherichia coli: a novel method of ethyl vanillin synthesis (2013), Biotechnol. Lett., 35, 921-927.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.1.7 | synthesis | the enzyme recombinantly expressed in Escherichia coli is useful for production of ethyl vanillin, i.e. 3-ethoxy-4-hydroxybenzaldehyde, which is a good substitute for vanillin in foods and perfumes since it is 3.5times stronger in flavor and keeps better condition in storage and transport | Pseudomonas putida |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.7 | gene mdlC, recombinant expression in Escherichia coli strain BL21(DE3), suncloning in Escherichia coli strain DH5alpha | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.1.7 | additional information | biotransformation using recycled resting Escherichia coli cells expressing the enzyme, the product yield is above 42% after four repeated cycles | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.7 | 3-ethoxy-4-hydroxybenzoylformate | Pseudomonas putida | - |
ethyl vanillin + CO2 | - |
? |  |
| 4.1.1.7 | 3-ethoxy-4-hydroxybenzoylformate | Pseudomonas putida ATCC 12633 | - |
ethyl vanillin + CO2 | - |
? | |
| 4.1.1.7 | additional information | Pseudomonas putida | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | ? | - |
? | |
| 4.1.1.7 | additional information | Pseudomonas putida ATCC 12633 | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | ? | - |
? | |
| 4.1.1.7 | phenylglyoxylate | Pseudomonas putida | - |
benzaldehyde + CO2 | - |
? | |
| 4.1.1.7 | phenylglyoxylate | Pseudomonas putida ATCC 12633 | - |
benzaldehyde + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.7 | Pseudomonas putida | P20906 | gene mdlC | - |
| 4.1.1.7 | Pseudomonas putida ATCC 12633 | P20906 | gene mdlC | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.7 | 3-ethoxy-4-hydroxybenzoylformate | - |
Pseudomonas putida | ethyl vanillin + CO2 | - |
? | |
| 4.1.1.7 | 3-ethoxy-4-hydroxybenzoylformate | - |
Pseudomonas putida ATCC 12633 | ethyl vanillin + CO2 | - |
? | |
| 4.1.1.7 | additional information | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | Pseudomonas putida | ? | - |
? | |
| 4.1.1.7 | additional information | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | Pseudomonas putida ATCC 12633 | ? | - |
? | |
| 4.1.1.7 | phenylglyoxylate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
| 4.1.1.7 | phenylglyoxylate | - |
Pseudomonas putida ATCC 12633 | benzaldehyde + CO2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.7 | 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.7 | 7 | - |
assay at | Pseudomonas putida |
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Release 2023.1 (January 2023)
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