EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.6 | expressed in Escherichia coli BL21(DE3) cells | Pseudothermotoga lettingae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 64 | - |
D-psicose | at pH 8.0 and 75°C | Pseudothermotoga lettingae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.6 | additional information | 1 mM Co2+, Mn2+, Mg2+, Ni2+, Zn2+, Ca2+, Cu2+, and EDTA do not influence enzyme activity | Pseudothermotoga lettingae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 16015 | - |
2 * 16015, calculated from amino acid sequence | Pseudothermotoga lettingae |
5.3.1.6 | 16800 | - |
2 * 16800, SDS-PAGE | Pseudothermotoga lettingae |
5.3.1.6 | 35200 | - |
gel filtration | Pseudothermotoga lettingae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-ribose 5-phosphate | Pseudothermotoga lettingae | - |
D-ribulose 5-phosphate | - |
? | |
5.3.1.6 | D-ribose 5-phosphate | Pseudothermotoga lettingae TMO | - |
D-ribulose 5-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.6 | Pseudothermotoga lettingae | - |
- |
- |
5.3.1.6 | Pseudothermotoga lettingae TMO | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.6 | Ni-affinity column chromatography | Pseudothermotoga lettingae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 0.2 | - |
after 9.1fold purification, at pH 8.0 and 75°C | Pseudothermotoga lettingae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-psicose | the enzyme convertes D-psicose to D-allose maximally at 75°C and pH 8.0 with a 32% conversion yield | Pseudothermotoga lettingae | D-allose | - |
? | |
5.3.1.6 | D-psicose | the enzyme convertes D-psicose to D-allose maximally at 75°C and pH 8.0 with a 32% conversion yield | Pseudothermotoga lettingae TMO | D-allose | - |
? | |
5.3.1.6 | D-ribose 5-phosphate | - |
Pseudothermotoga lettingae | D-ribulose 5-phosphate | - |
? | |
5.3.1.6 | D-ribose 5-phosphate | - |
Pseudothermotoga lettingae TMO | D-ribulose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.6 | homodimer | 2 * 16800, SDS-PAGE | Pseudothermotoga lettingae |
5.3.1.6 | homodimer | 2 * 16015, calculated from amino acid sequence | Pseudothermotoga lettingae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.6 | RPI | - |
Pseudothermotoga lettingae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 75 | - |
- |
Pseudothermotoga lettingae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 75 | - |
the half-life at 75°C is 3.3 h | Pseudothermotoga lettingae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 0.116 | - |
D-psicose | at pH 8.0 and 75°C | Pseudothermotoga lettingae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 8 | - |
- |
Pseudothermotoga lettingae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 6 | 9 | more than 80% of its maximal activity is maintained at pH values from 6.0 to 9.0 | Pseudothermotoga lettingae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 0.0018 | - |
D-psicose | at pH 8.0 and 75°C | Pseudothermotoga lettingae |