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Literature summary extracted from

  • Sakamoto, S.; Fujikawa, Y.; Tanaka, N.; Esaka, M.
    Molecular cloning and characterization of L-galactose-1-phosphate phosphatase from tobacco (Nicotiana tabacum) (2012), Biosci. Biotechnol. Biochem., 76, 1155-1162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.B9 expressed in Escherichia coli and in tobacco BY-2 cells Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.93 Ca2+
-
Nicotiana tabacum
3.1.3.93 Li+
-
Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.B9 cytosol
-
Nicotiana tabacum 5829
-
3.1.3.B9 nucleus
-
Nicotiana tabacum 5634
-
3.1.3.93 cytosol
-
Nicotiana tabacum 5829
-
3.1.3.93 nucleus
-
Nicotiana tabacum 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.B9 Ca2+ inhibits activity Nicotiana tabacum
3.1.3.B9 Li+ inhibits activity Nicotiana tabacum
3.1.3.B9 Mg2+ required Nicotiana tabacum
3.1.3.93 Mg2+ required Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.B9 58000
-
GST-NtGPPase fusion protein Nicotiana tabacum
3.1.3.93 58000
-
x * 58000, SDS-PAGE, fusion proein with glutathione S-transferase Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.B9 Nicotiana tabacum
-
-
-
3.1.3.93 Nicotiana tabacum B3GK03
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.B9 0.0035
-
substrate: myo-inositol 1-phosphate, pH 7.5, 30°C Nicotiana tabacum
3.1.3.B9 0.0376
-
substrate: L-galactose 1-phosphate, pH 7.5, 30°C Nicotiana tabacum
3.1.3.93 37.6
-
pH 7.5, 30°C Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.B9 L-galactose 1-phosphate + H2O
-
Nicotiana tabacum L-galactose + phosphate
-
?
3.1.3.B9 additional information enzyme also dephosphorylates myo-inositol 1-phosphate Nicotiana tabacum ?
-
?
3.1.3.93 beta-L-galactose 1-phosphate + H2O
-
Nicotiana tabacum L-galactose + phosphate
-
?
3.1.3.93 myo-inositol 1-phosphate + H2O 9% of the activity with beta-L-galactose Nicotiana tabacum myo-inositol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.93 ? x * 58000, SDS-PAGE, fusion proein with glutathione S-transferase Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.B9 L-galactose-1-phosphate phosphatase
-
Nicotiana tabacum
3.1.3.B9 NtGPPase
-
Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.B9 30
-
assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.B9 7.5
-
-
Nicotiana tabacum
3.1.3.93 7.5
-
-
Nicotiana tabacum

Expression

EC Number Organism Comment Expression
3.1.3.93 Nicotiana tabacum mRNA and protein expression are clearly correlated with L-ascorbic acid contents of cells during culture additional information

General Information

EC Number General Information Comment Organism
3.1.3.B9 malfunction tobacco BY-2 cells over-expressing NtGPPase have a higher content of L-ascorbic acid 13 days after subculture compared to control cells Nicotiana tabacum
3.1.3.93 physiological function the ascorbic acid contents of enzyme overexpression lines are higher than those of controls Nicotiana tabacum