EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.B9 | expressed in Escherichia coli and in tobacco BY-2 cells | Nicotiana tabacum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.93 | Ca2+ | - |
Nicotiana tabacum | |
3.1.3.93 | Li+ | - |
Nicotiana tabacum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.B9 | cytosol | - |
Nicotiana tabacum | 5829 | - |
3.1.3.B9 | nucleus | - |
Nicotiana tabacum | 5634 | - |
3.1.3.93 | cytosol | - |
Nicotiana tabacum | 5829 | - |
3.1.3.93 | nucleus | - |
Nicotiana tabacum | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.B9 | Ca2+ | inhibits activity | Nicotiana tabacum | |
3.1.3.B9 | Li+ | inhibits activity | Nicotiana tabacum | |
3.1.3.B9 | Mg2+ | required | Nicotiana tabacum | |
3.1.3.93 | Mg2+ | required | Nicotiana tabacum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.B9 | 58000 | - |
GST-NtGPPase fusion protein | Nicotiana tabacum |
3.1.3.93 | 58000 | - |
x * 58000, SDS-PAGE, fusion proein with glutathione S-transferase | Nicotiana tabacum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.B9 | Nicotiana tabacum | - |
- |
- |
3.1.3.93 | Nicotiana tabacum | B3GK03 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.B9 | 0.0035 | - |
substrate: myo-inositol 1-phosphate, pH 7.5, 30°C | Nicotiana tabacum |
3.1.3.B9 | 0.0376 | - |
substrate: L-galactose 1-phosphate, pH 7.5, 30°C | Nicotiana tabacum |
3.1.3.93 | 37.6 | - |
pH 7.5, 30°C | Nicotiana tabacum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.B9 | L-galactose 1-phosphate + H2O | - |
Nicotiana tabacum | L-galactose + phosphate | - |
? | |
3.1.3.B9 | additional information | enzyme also dephosphorylates myo-inositol 1-phosphate | Nicotiana tabacum | ? | - |
? | |
3.1.3.93 | beta-L-galactose 1-phosphate + H2O | - |
Nicotiana tabacum | L-galactose + phosphate | - |
? | |
3.1.3.93 | myo-inositol 1-phosphate + H2O | 9% of the activity with beta-L-galactose | Nicotiana tabacum | myo-inositol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.93 | ? | x * 58000, SDS-PAGE, fusion proein with glutathione S-transferase | Nicotiana tabacum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.B9 | L-galactose-1-phosphate phosphatase | - |
Nicotiana tabacum |
3.1.3.B9 | NtGPPase | - |
Nicotiana tabacum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.B9 | 30 | - |
assay at | Nicotiana tabacum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.B9 | 7.5 | - |
- |
Nicotiana tabacum |
3.1.3.93 | 7.5 | - |
- |
Nicotiana tabacum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.1.3.93 | Nicotiana tabacum | mRNA and protein expression are clearly correlated with L-ascorbic acid contents of cells during culture | additional information |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.B9 | malfunction | tobacco BY-2 cells over-expressing NtGPPase have a higher content of L-ascorbic acid 13 days after subculture compared to control cells | Nicotiana tabacum |
3.1.3.93 | physiological function | the ascorbic acid contents of enzyme overexpression lines are higher than those of controls | Nicotiana tabacum |