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Literature summary extracted from

  • Smith, N.E.; Vrielink, A.; Attwood, P.V.; Corry, B.
    Binding and channeling of alternative substrates in the enzyme DmpFG: a molecular dynamics study (2014), Biophys. J., 106, 1681-1690.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.10 I159A site-directed mutagenesis, the barrier into the dehydrogenase active site region has been virtually eliminated such that acetaldehyde is transported from one active site to the other in a downhill process Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 additional information Pseudomonas sp. DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview ?
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?
1.2.1.10 additional information Pseudomonas sp. CF 600 DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview ?
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?
4.1.3.39 4-hydroxy-2-oxovalerate Pseudomonas sp. physiological substrate acetaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxovalerate Pseudomonas sp. CF 600 physiological substrate acetaldehyde + pyruvate
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?
4.1.3.39 additional information Pseudomonas sp. DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview ?
-
?
4.1.3.39 additional information Pseudomonas sp. CF 600 DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Pseudomonas sp. Q52060 dmpF
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1.2.1.10 Pseudomonas sp. CF 600 Q52060 dmpF
-
4.1.3.39 Pseudomonas sp. P51016 dmpG
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4.1.3.39 Pseudomonas sp. CF 600 P51016 dmpG
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 additional information DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview Pseudomonas sp. ?
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?
1.2.1.10 additional information DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview Pseudomonas sp. CF 600 ?
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?
4.1.3.39 4-hydroxy-2-oxo-4-benzylbutanoate
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Pseudomonas sp. benzaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxo-isoheptanoate
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Pseudomonas sp. isobutyraldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxoheptanoate
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Pseudomonas sp. butyraldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxohexanoate
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Pseudomonas sp. propionaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxohexanoate
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Pseudomonas sp. CF 600 propionaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxovalerate physiological substrate Pseudomonas sp. acetaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxovalerate physiological substrate, the R and the S forms are both accommodated within the DmpG active site Pseudomonas sp. acetaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxovalerate physiological substrate Pseudomonas sp. CF 600 acetaldehyde + pyruvate
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?
4.1.3.39 4-hydroxy-2-oxovalerate physiological substrate, the R and the S forms are both accommodated within the DmpG active site Pseudomonas sp. CF 600 acetaldehyde + pyruvate
-
?
4.1.3.39 additional information DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview Pseudomonas sp. ?
-
?
4.1.3.39 additional information substrate specificity, substrate docking into the active site and modeling, binding structures, overview Pseudomonas sp. ?
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?
4.1.3.39 additional information DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview Pseudomonas sp. CF 600 ?
-
?
4.1.3.39 additional information substrate specificity, substrate docking into the active site and modeling, binding structures, overview Pseudomonas sp. CF 600 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.10 More DmpFG is a microbial enzyme comprised of two subunits DmpG and DmpF Pseudomonas sp.
4.1.3.39 More DmpFG is a microbial enzyme comprised of two subunits DmpG and DmpF Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.10 DmpFG
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Pseudomonas sp.
4.1.3.39 4-hydroxy-2-ketovalerate aldolase-aldehyde dehydrogenase (acylation)
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Pseudomonas sp.
4.1.3.39 DmpFG
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Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.2.1.10 metabolism DmpFG catalyzes the final two steps of the meta-cleavage pathway of catechol and its methylated substituents. This pathway breaks down toxic waste products such as naphthalenes, salicylates, and benzoates to harmless metabolites Pseudomonas sp.
4.1.3.39 metabolism DmpFG catalyzes the final two steps of the meta-cleavage pathway of catechol and its methylated substituents. This pathway breaks down toxic waste products such as naphthalenes, salicylates, and benzoates to harmless metabolites Pseudomonas sp.