EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.10 | I159A | site-directed mutagenesis, the barrier into the dehydrogenase active site region has been virtually eliminated such that acetaldehyde is transported from one active site to the other in a downhill process | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.10 | additional information | Pseudomonas sp. | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | ? | - |
? | |
1.2.1.10 | additional information | Pseudomonas sp. CF 600 | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | ? | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | Pseudomonas sp. | physiological substrate | acetaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | Pseudomonas sp. CF 600 | physiological substrate | acetaldehyde + pyruvate | - |
? | |
4.1.3.39 | additional information | Pseudomonas sp. | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | ? | - |
? | |
4.1.3.39 | additional information | Pseudomonas sp. CF 600 | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.10 | Pseudomonas sp. | Q52060 | dmpF | - |
1.2.1.10 | Pseudomonas sp. CF 600 | Q52060 | dmpF | - |
4.1.3.39 | Pseudomonas sp. | P51016 | dmpG | - |
4.1.3.39 | Pseudomonas sp. CF 600 | P51016 | dmpG | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.10 | additional information | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | Pseudomonas sp. | ? | - |
? | |
1.2.1.10 | additional information | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | Pseudomonas sp. CF 600 | ? | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxo-4-benzylbutanoate | - |
Pseudomonas sp. | benzaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxo-isoheptanoate | - |
Pseudomonas sp. | isobutyraldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxoheptanoate | - |
Pseudomonas sp. | butyraldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxohexanoate | - |
Pseudomonas sp. | propionaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxohexanoate | - |
Pseudomonas sp. CF 600 | propionaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | physiological substrate | Pseudomonas sp. | acetaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | physiological substrate, the R and the S forms are both accommodated within the DmpG active site | Pseudomonas sp. | acetaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | physiological substrate | Pseudomonas sp. CF 600 | acetaldehyde + pyruvate | - |
? | |
4.1.3.39 | 4-hydroxy-2-oxovalerate | physiological substrate, the R and the S forms are both accommodated within the DmpG active site | Pseudomonas sp. CF 600 | acetaldehyde + pyruvate | - |
? | |
4.1.3.39 | additional information | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | Pseudomonas sp. | ? | - |
? | |
4.1.3.39 | additional information | substrate specificity, substrate docking into the active site and modeling, binding structures, overview | Pseudomonas sp. | ? | - |
? | |
4.1.3.39 | additional information | DmpFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, DmpF. The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecular channel in the protein structure from the aldolase to the dehydrogenase active site. Binding and channeling of alternative substrates in the enzyme DmpFG, molecular dynamics, overview | Pseudomonas sp. CF 600 | ? | - |
? | |
4.1.3.39 | additional information | substrate specificity, substrate docking into the active site and modeling, binding structures, overview | Pseudomonas sp. CF 600 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.10 | More | DmpFG is a microbial enzyme comprised of two subunits DmpG and DmpF | Pseudomonas sp. |
4.1.3.39 | More | DmpFG is a microbial enzyme comprised of two subunits DmpG and DmpF | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.10 | DmpFG | - |
Pseudomonas sp. |
4.1.3.39 | 4-hydroxy-2-ketovalerate aldolase-aldehyde dehydrogenase (acylation) | - |
Pseudomonas sp. |
4.1.3.39 | DmpFG | - |
Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.1.10 | metabolism | DmpFG catalyzes the final two steps of the meta-cleavage pathway of catechol and its methylated substituents. This pathway breaks down toxic waste products such as naphthalenes, salicylates, and benzoates to harmless metabolites | Pseudomonas sp. |
4.1.3.39 | metabolism | DmpFG catalyzes the final two steps of the meta-cleavage pathway of catechol and its methylated substituents. This pathway breaks down toxic waste products such as naphthalenes, salicylates, and benzoates to harmless metabolites | Pseudomonas sp. |