Literature summary extracted from
Sasikumar, P.; Gomathi, S.; Anbazhagan, K.; Selvam, G.S.
Secretion of biologically active heterologous oxalate decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 using homologous signal peptides (2013), BioMed Res. Int., 2013, 280432.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.1.1.2 |
medicine |
the transgenic Lactobacillus plantarum strain WCFS1 expressing the enzyme from Bacillus subtilis may provide possible therapeutic approach by degrading intestinal oxalate in the human host of Lactobacillus plantarum |
Bacillus subtilis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.1.2 |
gene oxdC, cloning in Escherichia coli strain DH10B, functional overexpression of His-tagged enzyme in Lactobacillus plantarum strain WCFS1, isolated from human saliva, using homologous signal peptides Lp 0373 and Lp 3050, the recombinant strain secretes the recombinant protein. The recombinant Lactobacillus plantarum harboring pLp 0373sOxdC and pLp 3050sOxdC can express and secrete functional OxdC and degrade oxalate up to 50% and 30%, respectively |
Bacillus subtilis |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.1.2 |
Mn2+ |
required |
Bacillus subtilis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.1.1.2 |
44000 |
- |
x * 44000, recombinant His-tagged enzyme, SDS-PAGE |
Bacillus subtilis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.1.1.2 |
oxalate + H+ |
Bacillus subtilis |
- |
formate + CO2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.2 |
Bacillus subtilis |
- |
gene oxdC |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.1.2 |
recombinant His-tagged enzyme from Lactobacillus plantarum strain WCFS1 supernatant by ammonium sulfate fractionation, affinity chromatography, and dialysis |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.1.1.2 |
17.5 |
18.3 |
purified secreted recombinant enzyme, pH 4.0, 25°C |
Bacillus subtilis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.2 |
oxalate + H+ |
- |
Bacillus subtilis |
formate + CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.1.2 |
? |
x * 44000, recombinant His-tagged enzyme, SDS-PAGE |
Bacillus subtilis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.2 |
OXDC |
- |
Bacillus subtilis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.1.1.2 |
25 |
- |
assay at |
Bacillus subtilis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.1.1.2 |
4 |
- |
assay at |
Bacillus subtilis |