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Literature summary extracted from

  • Meng, D.; Shen, L.; Yang, R.; Zhang, X.; Sheng, J.
    Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus (2014), Biochim. Biophys. Acta, 1840, 120-128.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 bicarbonate the enzyme is completely dependent on bicarbonate Agaricus bisporus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 expressed in Escherichia coli BL21(DE3) cells Agaricus bisporus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.17.4 G289S the enzyme activity of the mutant is 3times as high as that of the wild type enzyme Agaricus bisporus
1.14.17.4 H216D the mutant shows 76% of wild type activity Agaricus bisporus
1.14.17.4 H216D/D218E the mutant shows 78% of wild type activity Agaricus bisporus
1.14.17.4 H216D/D218E/H273Q the mutant does not completely lose catalytic activity Agaricus bisporus
1.14.17.4 H273Q the mutant shows 62% of wild type activity Agaricus bisporus
1.14.17.4 R287G the activity of the mutant is remarkably decreased to nearly 40% compared to the wild type enzyme Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.25
-
 1-aminocyclopropane-1-carboxylate at pH and °C Agaricus bisporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+ in the absence of Fe2+, the activity is 2.4fold lower than that in the steady-state reaction when 0.0025 mM Fe2+ is added (maximum activity at 0.01 mM Fe2+) Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Agaricus bisporus
-
 ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Agaricus bisporus H9ZYN5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.4 fruit body
-
 Agaricus bisporus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Agaricus bisporus ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.4 1-aminocyclopropane-1-carboxylic acid oxidase
-
 Agaricus bisporus
1.14.17.4 ACC oxidase
-
 Agaricus bisporus
1.14.17.4 ACO
-
 Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.4 additional information the enzyme does not require ascorbate Agaricus bisporus