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Literature summary extracted from

  • Rangaswamy, V.; Altekar, W.
    Characterization of 1-phosphofructokinase from halophilic archaebacterium Haloarcula vallismortis (1994), Biochim. Biophys. Acta, 1201, 106-112.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.56 reduced glutathione serves as the best sulphydryl compound for optimum activity. Cysteine, 2-mercaptoethanol and dithiothreitol can substitute for glutathione contributing about 80% of the activity Haloarcula vallismortis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.56 4-chloromercuribenzoate
-
 Haloarcula vallismortis
2.7.1.56 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Haloarcula vallismortis
2.7.1.56 ADP competitive Haloarcula vallismortis
2.7.1.56 DTNB
-
 Haloarcula vallismortis
2.7.1.56 LiCl
-
 Haloarcula vallismortis
2.7.1.56 NH4Cl activation below 0.8 mM, inhibition above 0.8 M Haloarcula vallismortis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.56 0.8
-
 D-Fructose 1-phosphate pH 7.5, 37°C Haloarcula vallismortis
2.7.1.56 1.44
-
 ATP pH 7.5, 37°C Haloarcula vallismortis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 CsCl stimulates Haloarcula vallismortis
2.7.1.56 KCl stimulates, optimal concentration: 1.2 M Haloarcula vallismortis
2.7.1.56 Mg2+ is the most active divalent cation and can not be replaced either by Mn2+ or any other divalent cation. The optimal ratio of ATP/Mg2+ is 1:1 Haloarcula vallismortis
2.7.1.56 NaCl 50% of the stimulation compared to KCl Haloarcula vallismortis
2.7.1.56 NH4Cl activation below 0.8 mM, inhibition above 0.8 M Haloarcula vallismortis
2.7.1.56 RbCl stimulates Haloarcula vallismortis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.56 76000
-
 gel filtration, sucrose density gradient ultracentrifugation Haloarcula vallismortis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.56 Haloarcula vallismortis
-
-
-
2.7.1.56 Haloarcula vallismortis ATCC 34679
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.56
-
 Haloarcula vallismortis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate
-
 Haloarcula vallismortis ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ATP + D-fructose 1-phosphate
-
 Haloarcula vallismortis ATCC 34679 ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 CTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 CTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 GTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 GTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 TTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis TDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 TTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 TDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 UTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis UDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 UTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 UDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.56 monomer SDS-PAGE Haloarcula vallismortis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.56 1PFK
-
 Haloarcula vallismortis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.56 37
-
 assay at Haloarcula vallismortis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.56 25
-
 pH 9.0, 0.1 M KCl, 50 mM Tris-HCl, 80% loss of activity after 2 h. The enzyme is fully active for more than 2 months in 2.5 M KCl/50 mM Tris-HCl buffer, pH 9.0. Phosphate at 50 mM concentration stabilizes the enzyme against inactivation in 0.1 M KCI for about 24 h. (NH4)2SO4 though inhibitory for expression of the enzyme activity is stabilizing Haloarcula vallismortis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.56 10.7
-
 steady increase in activity from pH 7.5 to 10.7. Activity at pHs above 10.7 are not tested due to limitations of the assay system Haloarcula vallismortis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.56 7.5 10.7 steady increase in activity from pH 7.5 to 10.7. Activity at pHs above 10.7 are not tested due to limitations of the assay system Haloarcula vallismortis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.56 0.68
-
 ADP pH 7.5, 37°C Haloarcula vallismortis