EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | ATP | ATP maximally stimulates CDP reduction at 1.5 mM | Bacillus subtilis | |
1.17.4.1 | dATP | dATP maximally stimulates CDP reduction at 8-10 microM followed by rapid inhibition at higher concentrations | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | dATP | dATP maximally stimulates CDP reduction at 8-10 microM followed by rapid inhibition at higher concentrations | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | Iron | may substitute or manganese | Bacillus subtilis | |
1.17.4.1 | Manganese | dimanganic-tyrosyl radical cofactor | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.4.1 | 49600 | - |
isolated subunit NrdF, plus major fraction of 80100 Dalton, gel filtration | Bacillus subtilis |
1.17.4.1 | 80100 | - |
isolated subunit NrdF, plus small fraction of 49600 Dalton, gel filtration | Bacillus subtilis |
1.17.4.1 | 82700 | - |
isolated subunit NrdE | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.4.1 | Bacillus subtilis | - |
- |
- |
1.17.4.1 | Bacillus subtilis JH624 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.4.1 | isolation of beta-subunit NrdF using anion exchange chromatography to separate apo-/mismetalated-NrdFs from dimanganic-tyrosyl radical cofactor | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.4.1 | 52 | - |
iron-loaded enzyme, cosubstrate dithiothreitol, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
1.17.4.1 | 106 | - |
iron-loaded enzyme, cosubstrate thioredoxin YosR, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
1.17.4.1 | 125 | - |
iron-loaded enzyme, cosubstrate thioredoxin A, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
1.17.4.1 | 146 | - |
manganese-loaded enzyme, cosubstrate dithiothreitol, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
1.17.4.1 | 997 | - |
manganese-loaded enzyme, cosubstrate thioredoxin YosR, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
1.17.4.1 | 1475 | - |
manganese-loaded enzyme, cosubstrate thioredoxin A, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.4.1 | CDP + dithiothreitol | - |
Bacillus subtilis | 2'-dCDP + oxidized dithiothreitol + H2O | - |
? | |
1.17.4.1 | CDP + dithiothreitol | - |
Bacillus subtilis JH624 | 2'-dCDP + oxidized dithiothreitol + H2O | - |
? | |
1.17.4.1 | CDP + thioredoxin A | - |
Bacillus subtilis | 2'-dCDP + thioredoxin A disulfide + H2O | - |
? | |
1.17.4.1 | CDP + thioredoxin A | - |
Bacillus subtilis JH624 | 2'-dCDP + thioredoxin A disulfide + H2O | - |
? | |
1.17.4.1 | CDP + thioredoxin YosR | - |
Bacillus subtilis | 2'-dCDP + thioredoxin YosR disulfide + H2O | - |
? | |
1.17.4.1 | CDP + thioredoxin YosR | - |
Bacillus subtilis JH624 | 2'-dCDP + thioredoxin YosR disulfide + H2O | - |
? | |
1.17.4.1 | additional information | Bacillus subtilis ribonucleotide reductase can be assayed as a holo-enzyme by using equivalent amounts of each subunit | Bacillus subtilis | ? | - |
? | |
1.17.4.1 | additional information | Bacillus subtilis ribonucleotide reductase can be assayed as a holo-enzyme by using equivalent amounts of each subunit | Bacillus subtilis JH624 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.4.1 | More | at physiological concentrations, alpha-subunit NrdE is a monomer and beta-subunit NrdF in complex with dimanganic-tyrosyl radical cofactor is a dimer. A 1:1 mixture of NrdE:NrdF, however, is composed of a complex mixture of structures | Bacillus subtilis |