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Literature summary extracted from

  • Vinokur, J.M.; Korman, T.P.; Cao, Z.; Bowie, J.U.
    Evidence of a novel mevalonate pathway in archaea (2014), Biochemistry, 53, 4161-4168.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.185 expression in Escherichia coli Thermoplasma acidophilum
2.7.1.186 expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.185 0.097
-
 (R)-mevalonate pH 8.5, 55°C Thermoplasma acidophilum
2.7.1.186 additional information
-
 (R)-3-phosphomevalonate pH 8.0, 60°C, it is not possible to accurately determine the Km for mevalonate-3-phosphate-5-kinase, however, because the substrate is not commercially available and the detection limit of the assay is 0.03 mM substrate. The enzyme is still at Vmax when it is assayed with 0.06 mM mevalonate 3-phosphate, indicating that the Km is well below 0.06 mM Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.185 ATP + (R)-mevalonate Thermoplasma acidophilum mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum ADP + (R)-3-phosphomevalonate
-
 ?
2.7.1.186 ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum ADP + mevalonate 3,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.185 Thermoplasma acidophilum Q9HIN1
-
-
2.7.1.186 Thermoplasma acidophilum Q9HK44
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.185
-
 Thermoplasma acidophilum
2.7.1.186
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.185 ATP + (R)-mevalonate
-
 Thermoplasma acidophilum ADP + (R)-3-phosphomevalonate
-
 ?
2.7.1.185 ATP + (R)-mevalonate mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum ADP + (R)-3-phosphomevalonate
-
 ?
2.7.1.186 ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum ADP + mevalonate 3,5-bisphosphate
-
 ?
2.7.1.186 ATP + (R)-3-phosphomevalonate mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum ADP + mevalonate 3,5-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.185 Ta1305 locus name Thermoplasma acidophilum
2.7.1.186 Ta0762 locus name Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.185 55
-
-
 Thermoplasma acidophilum
2.7.1.186 60
-
-
 Thermoplasma acidophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.185 40 60 40°C: about 55% of maximal activity, 60°C: about 95% of maximal activity Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.185 60
-
 1 h, enzyme retains more than 95% of its activity Thermoplasma acidophilum
2.7.1.185 70
-
 1 h, about 75% loss of activity Thermoplasma acidophilum
2.7.1.185 75
-
 1 h, complete loss of activity Thermoplasma acidophilum
2.7.1.186 60
-
 1 h, enzyme retains more than 95% of its activity Thermoplasma acidophilum
2.7.1.186 70
-
 1 h, about 75% loss of activity Thermoplasma acidophilum
2.7.1.186 80
-
 1 h, complete loss of activity Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.185 5
-
 (R)-mevalonate pH 8.5, 55°C Thermoplasma acidophilum
2.7.1.186 9
-
 (R)-3-phosphomevalonate pH 8.0, 60°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.185 8.5
-
-
 Thermoplasma acidophilum
2.7.1.186 6.5 9 no significant pH dependence in the pH range of 6.5-9.0 Thermoplasma acidophilum
2.7.1.186 8
-
 assay at Thermoplasma acidophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.185 7 9.5 pH 7.0: about 75% of maximal activity, pH 9.5: about 85% of maximal activity Thermoplasma acidophilum

General Information

EC Number General Information Comment Organism
2.7.1.185 physiological function mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum
2.7.1.186 physiological function mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.185 51.5
-
 (R)-mevalonate pH 8.5, 55°C Thermoplasma acidophilum