EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.9 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
3.2.2.16 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
3.2.2.16 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
3.2.2.16 | expressed in Escherichia coli BL21(DE3) cells | Vibrio cholerae |
3.2.2.30 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.2.16 | sitting drop vapor diffusion method | Vibrio cholerae |
3.2.2.16 | sitting drop vapor diffusion method, using | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.2.9 | E173Q | inactive | Staphylococcus aureus |
3.2.2.9 | F104C/C186S | the mutations give an enzyme with steady-state kinetic properties similar to the native enzyme | Staphylococcus aureus |
3.2.2.16 | A113P | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Vibrio cholerae |
3.2.2.16 | A113P/V153I | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Vibrio cholerae |
3.2.2.16 | A113P/V153I/R158G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Vibrio cholerae |
3.2.2.16 | E173Q | inactive | Staphylococcus aureus |
3.2.2.16 | F104C/C186S | the mutant shows reduced activity compared to the wild type | Staphylococcus aureus |
3.2.2.16 | V153I | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Vibrio cholerae |
3.2.2.30 | E173Q | inactive | Staphylococcus aureus |
3.2.2.30 | F104C/C186S | the mutations give an enzyme with steady-state kinetic properties similar to the native enzyme | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.9 | methylthio-DADMe-immucillin-A | i.e. (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol | Staphylococcus aureus | |
3.2.2.16 | (3R,4S)-1-[9-deazaadenin-(9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine | - |
Staphylococcus aureus | |
3.2.2.16 | 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
3.2.2.16 | 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Vibrio cholerae | |
3.2.2.30 | methylthio-DADMe-immucillin-A | i.e. (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.16 | 0.000061 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.000139 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.7 and 25°C | Escherichia coli | |
3.2.2.16 | 0.000202 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.000262 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.000262 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P/V153I, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.00043 | - |
S-methyl-5'-thioadenosine | mutant enzyme V153I, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.0009 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.2 and 25°C | Staphylococcus aureus | |
3.2.2.16 | 0.0029 | - |
S-methyl-5'-thioadenosine | mutant enzyme F104C/C186S, at pH 7.2 and 25°C | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | Staphylococcus aureus | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.16 | S-adenosyl-L-homocysteine + H2O | Staphylococcus aureus | - |
S-D-ribosyl-L-homocysteine + adenine | - |
ir | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | Staphylococcus aureus | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | Escherichia coli | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | Vibrio cholerae | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | Staphylococcus aureus | - |
dehypoxanthine futalosine + adenine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.9 | Staphylococcus aureus | - |
- |
- |
3.2.2.16 | Escherichia coli | P0AF12 | - |
- |
3.2.2.16 | Staphylococcus aureus | Q99TQ0 | - |
- |
3.2.2.16 | Vibrio cholerae | A5F5R2 | - |
- |
3.2.2.30 | Staphylococcus aureus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.9 | Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
3.2.2.16 | Ni-NTA column chromatography | Escherichia coli |
3.2.2.16 | Ni-NTA column chromatography | Vibrio cholerae |
3.2.2.16 | Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
3.2.2.30 | Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Staphylococcus aureus | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.16 | S-adenosyl-L-homocysteine + H2O | - |
Staphylococcus aureus | S-D-ribosyl-L-homocysteine + adenine | - |
ir | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | - |
Staphylococcus aureus | S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | - |
Escherichia coli | S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | - |
Vibrio cholerae | S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.30 | 5,5'-dithiobis-2-nitrobenzoic acid + H2O | - |
Staphylococcus aureus | ? | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | - |
Staphylococcus aureus | dehypoxanthine futalosine + adenine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.9 | homodimer | - |
Staphylococcus aureus |
3.2.2.16 | homodimer | - |
Staphylococcus aureus |
3.2.2.30 | homodimer | - |
Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.9 | methylthioadenosine nucleosidase | - |
Staphylococcus aureus |
3.2.2.9 | MTAN | - |
Staphylococcus aureus |
3.2.2.16 | 5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase | - |
Escherichia coli |
3.2.2.16 | 5'-methylthioadenosine/S-adenosyl-L-homocysteine nucleosidase | - |
Vibrio cholerae |
3.2.2.16 | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Staphylococcus aureus |
3.2.2.16 | methylthioadenosine nucleosidase | - |
Escherichia coli |
3.2.2.16 | methylthioadenosine nucleosidase | - |
Vibrio cholerae |
3.2.2.16 | MTAN | - |
Staphylococcus aureus |
3.2.2.16 | MTAN | - |
Escherichia coli |
3.2.2.16 | MTAN | - |
Vibrio cholerae |
3.2.2.30 | methylthioadenosine nucleosidase | - |
Staphylococcus aureus |
3.2.2.30 | MTAN | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.16 | 0.0079 | - |
S-methyl-5'-thioadenosine | mutant enzyme V153I, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.074 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P/V153I, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.11 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.17 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.18 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 0.32 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.7 and 25°C | Escherichia coli | |
3.2.2.16 | 7.2 | - |
S-methyl-5'-thioadenosine | mutant enzyme F104C/C186S, at pH 7.2 and 25°C | Staphylococcus aureus | |
3.2.2.16 | 10.2 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.2 and 25°C | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.16 | 18 | - |
S-methyl-5'-thioadenosine | mutant enzyme V153I, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 200 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 400 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P/V153I, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 700 | - |
S-methyl-5'-thioadenosine | mutant enzyme A113P, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 2300 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.7 and 25°C | Escherichia coli | |
3.2.2.16 | 2480 | - |
S-methyl-5'-thioadenosine | mutant enzyme F104C/C186S, at pH 7.2 and 25°C | Staphylococcus aureus | |
3.2.2.16 | 3000 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.7 and 25°C | Vibrio cholerae | |
3.2.2.16 | 11300 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.2 and 25°C | Staphylococcus aureus |