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Literature summary extracted from

  • Smith, S.M.; Rawat, S.; Telser, J.; Hoffman, B.M.; Stemmler, T.L.; Rosenzweig, A.C.
    Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M (2011), Biochemistry, 50, 10231-10240.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.18.3 sitting drop vapor diffusion method, using 0.1 M MES, pH 6.5, 18% (w/v) PEG 750 MME, 0.03 M glycyl glycine, 0.01 M ZnSO4, at 22°C Methylocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.3 cyanide cyanide treatment of the enzyme removes about 98% of the copper and about 49% of the iron and abolishes all activity Methylocystis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane
-
Methylocystis sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 copper the enzyme contains about 2.3 copper ions per 100 kDa protomer the enzyme contains a mixture of Cu+ and Cu2+ Methylocystis sp.
1.14.18.3 Fe2+ the enzyme contains about 0.6 Fe2+ ions per 100 kDa protomer Methylocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2 Methylocystis sp.
-
methanol + acceptor + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylocystis sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.3 0.0053
-
using propylene as substrate, pH and temperature not specified in the publication Methylocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
Methylocystis sp. methanol + acceptor + H2O
-
?
1.14.18.3 Mn2+ + H2O2
-
Methylocystis sp. ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 particulate methane monooxygenase
-
Methylocystis sp.
1.14.18.3 pMMO
-
Methylocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.3 NADH
-
Methylocystis sp.