EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.18.3 | sitting drop vapor diffusion method, using 0.1 M MES, pH 6.5, 18% (w/v) PEG 750 MME, 0.03 M glycyl glycine, 0.01 M ZnSO4, at 22°C | Methylocystis sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.3 | cyanide | cyanide treatment of the enzyme removes about 98% of the copper and about 49% of the iron and abolishes all activity | Methylocystis sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.18.3 | membrane | - |
Methylocystis sp. | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.3 | copper | the enzyme contains about 2.3 copper ions per 100 kDa protomer the enzyme contains a mixture of Cu+ and Cu2+ | Methylocystis sp. | |
1.14.18.3 | Fe2+ | the enzyme contains about 0.6 Fe2+ ions per 100 kDa protomer | Methylocystis sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.3 | methane + reduced acceptor + H* + O2 | Methylocystis sp. | - |
methanol + acceptor + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.3 | Methylocystis sp. | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.18.3 | 0.0053 | - |
using propylene as substrate, pH and temperature not specified in the publication | Methylocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.3 | methane + reduced acceptor + H* + O2 | - |
Methylocystis sp. | methanol + acceptor + H2O | - |
? | |
1.14.18.3 | Mn2+ + H2O2 | - |
Methylocystis sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.3 | particulate methane monooxygenase | - |
Methylocystis sp. |
1.14.18.3 | pMMO | - |
Methylocystis sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.3 | NADH | - |
Methylocystis sp. |