EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.99.20 | Cu2+ | - |
Oryctolagus cuniculus | |
2.4.99.20 | additional information | the cyclization reaction is insensitive to free Ca2+ concentration in the range 20 nM100 microM. Mg2+ is not inhibitory | Oryctolagus cuniculus | |
2.4.99.20 | NAD+ | - |
Oryctolagus cuniculus | |
2.4.99.20 | NADP+ | - |
Oryctolagus cuniculus | |
2.4.99.20 | nicotinamide | inhibition of the cyclization reaction and is 30fold more potent at suppressing the base-exchange reaction. Inhibition is not via a competition with the nicotinic acid-binding site | Oryctolagus cuniculus | |
2.4.99.20 | Zn2+ | - |
Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.99.20 | 0.0334 | - |
NGDP+ | pH 7.4, temperature not specified in the publication | Oryctolagus cuniculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.99.20 | membrane | of heavy sarcoplasmic reticulum | Oryctolagus cuniculus | 16020 | - |
2.4.99.20 | sarcoplasmic reticulum | membrane of heavy sarcoplasmic reticulum | Oryctolagus cuniculus | 16529 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.99.20 | Oryctolagus cuniculus | - |
skeletal muscle isoform | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.20 | 2'-phospho-cyclic ADP-ribose + nicotinate | - |
Oryctolagus cuniculus | nicotinate-adenine dinucleotide phosphate | - |
? | |
2.4.99.20 | additional information | the cyclization reaction to form cADPr and cGDPr as well as the base-exchange reaction to form nicotinic acid adenine dinucleotide phosphate are strictly dependent on pH. Although the formation of cyclic GDP-ribose is optimized at pH 6, the synthesis of nicotinic acid adenine dinucleotide phosphate is most pronounced at a pH below 5. Nicotinic acid has virtually no influence on the cyclization reaction, but increases the affinity of NADP at an acidic pH and has the opposite effect at alkaline pH | Oryctolagus cuniculus | ? | - |
? | |
2.4.99.20 | NADP+ | - |
Oryctolagus cuniculus | 2'-phospho-cyclic ADP-ribose + nicotinamide | - |
? | |
2.4.99.20 | NGDP+ | - |
Oryctolagus cuniculus | 2'-phospho-cyclic GDP-ribose + nicotinamide | - |
? |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.4.99.20 | 0.00027 | - |
formation of cGDPr, pH 7.4, temperature not specified in the publication | Oryctolagus cuniculus | Cu2+ | |
2.4.99.20 | 0.0013 | - |
formation of cGDPr, pH 7.4, temperature not specified in the publication | Oryctolagus cuniculus | NADP+ | |
2.4.99.20 | 0.0023 | - |
formation of cGDPr, pH 7.4, temperature not specified in the publication | Oryctolagus cuniculus | Zn2+ | |
2.4.99.20 | 0.0054 | - |
formation of cGDPr, pH 7.4, temperature not specified in the publication | Oryctolagus cuniculus | NAD+ | |
2.4.99.20 | 0.7 | - |
cyclization reaction, pH 7.4, temperature not specified in the publication | Oryctolagus cuniculus | nicotinamide |