EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.1 | 0.02 | - |
CoA | pH 8.4, 80°C | Thermococcus guaymasensis | |
1.2.7.1 | 0.25 | - |
pyruvate | pH 8.4, 80°C | Thermococcus guaymasensis | |
4.1.1.1 | 0.25 | - |
pyruvate | pH 8.4, 80°C | Thermococcus guaymasensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | Mg2+ | dependent on | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 12000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
1.2.7.1 | 26000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
1.2.7.1 | 35000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
1.2.7.1 | 46000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
1.2.7.1 | 258000 | - |
gel filtration | Thermococcus guaymasensis |
4.1.1.1 | 12000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
4.1.1.1 | 26000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
4.1.1.1 | 35000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
4.1.1.1 | 46000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
4.1.1.1 | 258000 | - |
gel filtration | Thermococcus guaymasensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | Thermococcus guaymasensis | - |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | Thermococcus guaymasensis DSM 11113 | - |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
4.1.1.1 | pyruvate | Thermococcus guaymasensis | - |
acetaldehyde + CO2 | - |
? | |
4.1.1.1 | pyruvate | Thermococcus guaymasensis DSM 11113 | - |
acetaldehyde + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.1 | Sus scrofa | - |
- |
- |
1.2.7.1 | Thermococcus guaymasensis | W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 | W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta | - |
1.2.7.1 | Thermococcus guaymasensis DSM 11113 | W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 | W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta | - |
4.1.1.1 | Thermococcus guaymasensis | W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 | W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta | - |
4.1.1.1 | Thermococcus guaymasensis DSM 11113 | W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 | W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.7.1 | oxygen-sensitive enzyme, the t1/2 for the enzyme activity in 50 mM Tris-HCl, pH 7.8 containing 2 mM sodium dithionite and 2 mM DTT is approximately 30 min | Thermococcus guaymasensis |
4.1.1.1 | oxygen-sensitive enzyme, the t1/2 for the enzyme activity in 50 mM Tris-HCl, pH7.8 containing 2 mM sodium dithionite and 2 mM DTT is approximately 30 min | Thermococcus guaymasensis |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.1 | - |
Thermococcus guaymasensis |
4.1.1.1 | - |
Thermococcus guaymasensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 20.2 | - |
purified enzyme, pH 8.4, 80°C | Thermococcus guaymasensis |
4.1.1.1 | 3.8 | - |
purified enzyme, pH 8.4, 80°C | Thermococcus guaymasensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | - |
Thermococcus guaymasensis | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | - |
Thermococcus guaymasensis DSM 11113 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
4.1.1.1 | pyruvate | - |
Thermococcus guaymasensis | acetaldehyde + CO2 | - |
? | |
4.1.1.1 | pyruvate | - |
Thermococcus guaymasensis DSM 11113 | acetaldehyde + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.4.1 | heterotetramer | - |
Sus scrofa |
1.2.7.1 | octamer | 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
4.1.1.1 | octamer | 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.4.1 | PDH | - |
Sus scrofa |
1.2.7.1 | bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase | - |
Thermococcus guaymasensis |
1.2.7.1 | POR | - |
Thermococcus guaymasensis |
4.1.1.1 | bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase | - |
Thermococcus guaymasensis |
4.1.1.1 | PDC | - |
Thermococcus guaymasensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 85 | - |
- |
Sus scrofa |
1.2.7.1 | 95 | - |
above | Thermococcus guaymasensis |
4.1.1.1 | 85 | - |
- |
Thermococcus guaymasensis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 75 | 95 | 75°C: about 80% of maximal activity, 95°C: about 65% of maximal activity | Thermococcus guaymasensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 9.5 | - |
- |
Sus scrofa |
1.2.7.1 | 9.5 | - |
- |
Thermococcus guaymasensis |
4.1.1.1 | 9.5 | - |
- |
Thermococcus guaymasensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | NAD+ | required | Sus scrofa | |
1.2.4.1 | thiamine diphosphate | dependent on | Sus scrofa |