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Literature summary extracted from

  • Eram, M.S.; Oduaran, E.; Ma, K..
    The bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase from Thermococcus guaymasensis (2014), Archaea, 2014, 349379.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.02
-
 CoA pH 8.4, 80°C Thermococcus guaymasensis
1.2.7.1 0.25
-
 pyruvate pH 8.4, 80°C Thermococcus guaymasensis
4.1.1.1 0.25
-
 pyruvate pH 8.4, 80°C Thermococcus guaymasensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.4.1 Mg2+ dependent on Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 12000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
1.2.7.1 26000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
1.2.7.1 35000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
1.2.7.1 46000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
1.2.7.1 258000
-
 gel filtration Thermococcus guaymasensis
4.1.1.1 12000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
4.1.1.1 26000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
4.1.1.1 35000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
4.1.1.1 46000
-
 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
4.1.1.1 258000
-
 gel filtration Thermococcus guaymasensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Thermococcus guaymasensis
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Thermococcus guaymasensis DSM 11113
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
4.1.1.1 pyruvate Thermococcus guaymasensis
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Thermococcus guaymasensis DSM 11113
-
 acetaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Sus scrofa
-
-
-
1.2.7.1 Thermococcus guaymasensis W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta
-
1.2.7.1 Thermococcus guaymasensis DSM 11113 W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta
-
4.1.1.1 Thermococcus guaymasensis W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta
-
4.1.1.1 Thermococcus guaymasensis DSM 11113 W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 oxygen-sensitive enzyme, the t1/2 for the enzyme activity in 50 mM Tris-HCl, pH 7.8 containing 2 mM sodium dithionite and 2 mM DTT is approximately 30 min Thermococcus guaymasensis
4.1.1.1 oxygen-sensitive enzyme, the t1/2 for the enzyme activity in 50 mM Tris-HCl, pH7.8 containing 2 mM sodium dithionite and 2 mM DTT is approximately 30 min Thermococcus guaymasensis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1
-
 Thermococcus guaymasensis
4.1.1.1
-
 Thermococcus guaymasensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 20.2
-
 purified enzyme, pH 8.4, 80°C Thermococcus guaymasensis
4.1.1.1 3.8
-
 purified enzyme, pH 8.4, 80°C Thermococcus guaymasensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Thermococcus guaymasensis acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Thermococcus guaymasensis DSM 11113 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
4.1.1.1 pyruvate
-
 Thermococcus guaymasensis acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Thermococcus guaymasensis DSM 11113 acetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.4.1 heterotetramer
-
 Sus scrofa
1.2.7.1 octamer 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
4.1.1.1 octamer 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis

Synonyms

EC Number Synonyms Comment Organism
1.2.4.1 PDH
-
 Sus scrofa
1.2.7.1 bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase
-
 Thermococcus guaymasensis
1.2.7.1 POR
-
 Thermococcus guaymasensis
4.1.1.1 bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase
-
 Thermococcus guaymasensis
4.1.1.1 PDC
-
 Thermococcus guaymasensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.4.1 85
-
-
 Sus scrofa
1.2.7.1 95
-
 above Thermococcus guaymasensis
4.1.1.1 85
-
-
 Thermococcus guaymasensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.1 75 95 75°C: about 80% of maximal activity, 95°C: about 65% of maximal activity Thermococcus guaymasensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.4.1 9.5
-
-
 Sus scrofa
1.2.7.1 9.5
-
-
 Thermococcus guaymasensis
4.1.1.1 9.5
-
-
 Thermococcus guaymasensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.1 NAD+ required Sus scrofa
1.2.4.1 thiamine diphosphate dependent on Sus scrofa