EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.42 | gene IDP1, overexpression in Yarrowia lipolytica strain H355A, and coexpression with pyruvate carboxylase gene PYC1 | Yarrowia lipolytica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.42 | additional information | engineering the 2-oxoglutarate overproduction from raw glycerol by overexpression of the genes encoding NADP+-dependent isocitrate dehydrogenase and pyruvate carboxylase in Yarrowia lipolytica. Overexpression of NADP+-dependent isocitrate dehydrogenase IDP1 or simultaneous overexpression of IDP1 and pyruvate carboxylase gene PYC1 strongly increase the amount of secreted 2-oxoglutarate | Yarrowia lipolytica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.42 | Yarrowia lipolytica | Q6C2Y4 | - |
- |
1.1.1.42 | Yarrowia lipolytica E 150 | Q6C2Y4 | - |
- |
6.4.1.1 | Yarrowia lipolytica | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.42 | IDP1 | - |
Yarrowia lipolytica |
1.1.1.42 | NADP+-dependent isocitrate dehydrogenase | - |
Yarrowia lipolytica |
6.4.1.1 | Pyc1 | - |
Yarrowia lipolytica |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.4.1.1 | metabolism | simultaneous overexpression of isocitrate dehydrogenase (IDP1) and pyruvate carboxylase (PYC) gene PYC1 increase the amount of secreted alpha-ketoglutaric acid. About 19% more alpha-ketoglutaric acid in comparison with the control strain H355 are produced in bioreactor with raw glycerol as carbon source resulting in the highest yield of accumulated alpha-ketoglutaric acid of about 186 g/L after 117 h of cultivation | Yarrowia lipolytica |