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Literature summary extracted from

  • Zhu, S.; Song, D.; Gong, C.; Tang, P.; Li, X.; Wang, J.; Zheng, G.
    Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase (2013), Appl. Microbiol. Biotechnol., 97, 6769-6778.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.1 synthesis the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields Aeropyrum pernix
2.4.2.3 synthesis the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields Aeropyrum pernix

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression in Escherichia coli Aeropyrum pernix
2.4.2.3 expression in Escherichia coli, the native apUP protein without fusion tags is actively expressed, and about 50% of the protein is found in the soluble fraction of the cell lysate Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.318
-
 Inosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.1 0.578
-
 adenosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.1 0.69
-
 guanosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.3 0.248
-
 5-methyluridine pH 7.0, 90°C Aeropyrum pernix
2.4.2.3 0.615
-
 uridine pH 7.0, 90°C Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 30000
-
 gel filtration Aeropyrum pernix
2.4.2.3 30200
-
 6 * 30200, calculated from sequence Aeropyrum pernix
2.4.2.3 185300
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Aeropyrum pernix Q9YDC0
-
-
2.4.2.1 Aeropyrum pernix DSM 11879 Q9YDC0
-
-
2.4.2.3 Aeropyrum pernix Q9YA34
-
-
2.4.2.3 Aeropyrum pernix DSM 11879 Q9YA34
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Aeropyrum pernix
2.4.2.3
-
 Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxyadenosine + phosphate
-
 Aeropyrum pernix adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyadenosine + phosphate
-
 Aeropyrum pernix DSM 11879 adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2,6-diaminopurine nucleoside + phosphate
-
 Aeropyrum pernix alpha-D-ribose 1-phosphate + 2,6-diaminopurine
-
 ?
2.4.2.1 adenosine + phosphate
-
 Aeropyrum pernix adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate
-
 Aeropyrum pernix DSM 11879 adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Aeropyrum pernix guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Aeropyrum pernix DSM 11879 guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Aeropyrum pernix hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Aeropyrum pernix DSM 11879 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 additional information the enzyme cannot accept 6-chloropurines Aeropyrum pernix ?
-
 ?
2.4.2.1 additional information the enzyme cannot accept 6-chloropurines Aeropyrum pernix DSM 11879 ?
-
 ?
2.4.2.3 5-methyluridine + phosphate 92% compared to the activity with uridine Aeropyrum pernix 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 5-methyluridine + phosphate 92% compared to the activity with uridine Aeropyrum pernix DSM 11879 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 deoxyuridine + phosphate 66% compared to the activity with uridine Aeropyrum pernix uracil + deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.3 deoxyuridine + phosphate 66% compared to the activity with uridine Aeropyrum pernix DSM 11879 uracil + deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate 60% compared to the activity with uridine Aeropyrum pernix thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate 60% compared to the activity with uridine Aeropyrum pernix DSM 11879 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine Aeropyrum pernix uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine Aeropyrum pernix DSM 11879 uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 monomer 1 * 30000, SDS-PAGE Aeropyrum pernix
2.4.2.3 hexamer 6 * 30200, calculated from sequence Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 apPNP
-
 Aeropyrum pernix
2.4.2.3 apUP
-
 Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 100
-
-
 Aeropyrum pernix
2.4.2.3 90
-
-
 Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 100
-
 the recombinant protein has nearly 100% activity when incubated at 100°C for 60 min Aeropyrum pernix
2.4.2.3 90
-
 after incubation for 60 min at 90 °C, the enzyme remains 100% active Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 838
-
 Inosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.1 876
-
 guanosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.1 3450
-
 adenosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.3 56
-
 5-methyluridine pH 7.0, 90°C Aeropyrum pernix
2.4.2.3 150
-
 uridine pH 7.0, 90°C Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 5
-
-
 Aeropyrum pernix
2.4.2.3 9
-
-
 Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 3 11 the enzyme retains nearly 100% activity between pH 3.0 and 11.0 when incubated at 90 °C for 60 min Aeropyrum pernix

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.3 3 9 pH stability ranging from pH 3.0 to pH 9.0 Aeropyrum pernix

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 1270
-
 guanosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.1 2630
-
 Inosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.1 5997
-
 adenosine pH 7.0, 90°C Aeropyrum pernix
2.4.2.3 230
-
 5-methyluridine pH 7.0, 90°C Aeropyrum pernix
2.4.2.3 240
-
 uridine pH 7.0, 90°C Aeropyrum pernix