EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 1-butyl-3-methylimidazolium tetrafluoroborate | in the presence of 1-butyl-3-methylimidazolium tetrafluoroborate the selectivity of tyrosinase is altered, and the ratio of monophenolase/diphenolase activity increases by up to 5fold | Priestia megaterium | |
1.14.18.1 | ethylammonium nitrate | in the presence of ethylammonium nitrate the selectivity of tyrosinase is altered, and the ratio of monophenolase/diphenolase activity increases by up to 5fold | Priestia megaterium | |
1.14.18.1 | SDS | the addition of SDS at levels of 2-50 mM increases the activity of the enzyme by 2fold towards the natural substrates L-tyrosine and L-Dopa and 15-20fold towards the non-native phenol and catechol | Priestia megaterium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.18.1 | expressed in Escherichia coli BL21 cells | Priestia megaterium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.18.1 | - |
Priestia megaterium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.18.1 | R209H | the mutant has a 45fold increase in activity towards phenol in the presence of SDS compared to the wild type enzyme | Priestia megaterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 1-ethyl-3-methylimidazolium ethyl sulfate | in the presence of 10 or 20% (w/v) 1-ethyl-3-methylimidazolium ethyl sulfate, the activity decreases dramatically and becomes negligible | Priestia megaterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | copper | a type 3 copper protein | Priestia megaterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | L-Dopa + O2 | Priestia megaterium | - |
L-dopaquinone + H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | Priestia megaterium | - |
L-dopaquinone + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Priestia megaterium | B2ZB02 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | - |
Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 catechol + O2 | - |
Priestia megaterium | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.14.18.1 | L-Dopa + O2 | - |
Priestia megaterium | L-dopaquinone + H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | - |
Priestia megaterium | L-dopaquinone + H2O | - |
? | |
1.14.18.1 | phenol + O2 | - |
Priestia megaterium | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | diphenolase | - |
Priestia megaterium |
1.14.18.1 | monophenolase | - |
Priestia megaterium |
1.14.18.1 | tyr | - |
Priestia megaterium |