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Literature summary extracted from

  • Toda, H.; Imae, R.; Komio, T.; Itoh, N.
    Expression and characterization of styrene monooxygenases of Rhodococcus sp. ST-5 and ST-10 for synthesizing enantiopure (S)-epoxides (2012), Appl. Microbiol. Biotechnol., 96, 407-418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.11 expressed in Escherichia coli BL21(DE3) cells Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.11 3-chlorostyrene less than 30% residual activity with 25 mM 3-chlorostyrene; less than 30% residual activity with 25 mM 3-chlorostyrene Rhodococcus sp.
1.14.14.11 Benzene about 50% residual activity with 25 mM benzene; about 50% residual activity with 25 mM benzene Rhodococcus sp.
1.14.14.11 additional information not inhibited by 1-hexene; not inhibited by 1-hexene Rhodococcus sp.
1.14.14.11 Styrene less than 40% residual activity with 5 mM styrene; less than 40% residual activity with 5 mM styrene Rhodococcus sp.
1.14.14.11 styrene oxide marked inhibition; marked inhibition Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.11 45500
-
 1 * 45500, calculated from amino acid sequence Rhodococcus sp.
1.14.14.11 47300
-
 1 * 47300, calculated from amino acid sequence Rhodococcus sp.
1.14.14.11 59000
-
 gel filtration Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Rhodococcus sp.
-
 (S)-styrene oxide + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 Rhodococcus sp. ST-5
-
 (S)-styrene oxide + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Rhodococcus sp. G3XEX2
-
-
1.14.14.11 Rhodococcus sp. G3XEX5
-
-
1.14.14.11 Rhodococcus sp. ST-5 G3XEX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.11 Ni-Sepharose column chromatography and MonoQ column chromatography Rhodococcus sp.
1.14.14.11 Toyopearl SuperQ-650 M column chromatography, Ni-Sepharose column chromatography, MonoQ column chromatography and Superdex 200 gel filtration Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.11 0.0026
-
 cell-free extract, at pH 7.0 and 30°C Rhodococcus sp.
1.14.14.11 0.0114
-
 cell-free extract, at pH 7.0 and °C Rhodococcus sp.
1.14.14.11 0.0255
-
 after 2.2fold purification, at pH 7.0 and 30°C Rhodococcus sp.
1.14.14.11 0.0304
-
 after 11.7fold purification, at pH 7.0 and 30°C Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 1-hexene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 1-hexene + FADH2 + O2 highest activity Rhodococcus sp. ?
-
 ?
1.14.14.11 1-hexene + FADH2 + O2
-
 Rhodococcus sp. ST-5 ?
-
 ?
1.14.14.11 1-octene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 1-octene + FADH2 + O2
-
 Rhodococcus sp. ST-5 ?
-
 ?
1.14.14.11 2-chlorostyrene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 2-chlorostyrene + FADH2 + O2
-
 Rhodococcus sp. ST-5 ?
-
 ?
1.14.14.11 3-chlorostyrene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 3-chlorostyrene + FADH2 + O2
-
 Rhodococcus sp. ST-5 ?
-
 ?
1.14.14.11 4-bromostyrene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 4-chlorostyrene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 4-chlorostyrene + FADH2 + O2 highest activity Rhodococcus sp. ?
-
 ?
1.14.14.11 4-fluorostyrene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 allylbenzene + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 indole + FADH2 + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Rhodococcus sp. (S)-styrene oxide + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Rhodococcus sp. ST-5 (S)-styrene oxide + FAD + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.11 monomer 1 * 45500, calculated from amino acid sequence Rhodococcus sp.
1.14.14.11 monomer 1 * 47300, calculated from amino acid sequence Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.14.11 SmoA
-
 Rhodococcus sp.
1.14.14.11 StyA
-
 Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.11 FAD the enzyme favors FAD and FMN over riboflavin Rhodococcus sp.
1.14.14.11 FMN the enzyme favors FAD and FMN over riboflavin Rhodococcus sp.