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Literature summary extracted from

  • Nolte, J.C.; Schuermann, M.; Schepers, C.L.; Vogel, E.; Wuebbeler, J.H.; Steinbuechel, A.
    Novel characteristics of succinate coenzyme A (succinate-CoA) ligases: conversion of malate to malyl-CoA and CoA-thioester formation of succinate analogues in vitro (2014), Appl. Environ. Microbiol., 80, 166-176.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.5 gene sucCD, expression in Escherichia coli strain BL21(DE3)-pLysS Escherichia coli K-12
6.2.1.5 gene sucCD, expression in Escherichia coli strain BL21(DE3)-pLysS Advenella mimigardefordensis
6.2.1.5 gene sucCD, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)-pLysS Alcanivorax borkumensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.5 0.004
-
 CoA recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 0.037
-
 CoA recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 0.055
-
 ATP recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.058
-
 CoA recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.141
-
 succinate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.157
-
 succinate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 0.182
-
 succinate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 0.201
-
 ATP recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 0.241
-
 ATP recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 0.351
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 0.475
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 1.5 2 3-sulfinopropionate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 1.509
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 2.558
-
 L-malate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 2.964
-
 3-sulfinopropionate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 3.095
-
 L-malate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 3.27
-
 L-malate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 3.588
-
 D-malate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 3.635
-
 D-malate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 4.243
-
 D-malate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.5 ATP + itaconate + CoA Escherichia coli K-12
-
 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA Advenella mimigardefordensis
-
 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA Alcanivorax borkumensis
-
 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA Alcanivorax borkumensis SK2
-
 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA Advenella mimigardefordensis DPN7
-
 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA Escherichia coli K-12 BL21
-
 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA Advenella mimigardefordensis
-
 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA Alcanivorax borkumensis
-
 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA Escherichia coli K-12 ATP is the preferred cosubstrate ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA Alcanivorax borkumensis SK2
-
 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA Advenella mimigardefordensis DPN7
-
 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA Escherichia coli K-12 BL21 ATP is the preferred cosubstrate ADP + phosphate + succinyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.5 Advenella mimigardefordensis W0PAN5 alpha-subunit; gene sucCD
-
6.2.1.5 Advenella mimigardefordensis W0PFR9 beta-subunit; gene sucCD
-
6.2.1.5 Advenella mimigardefordensis DPN7 W0PAN5 alpha-subunit; gene sucCD
-
6.2.1.5 Advenella mimigardefordensis DPN7 W0PFR9 beta-subunit; gene sucCD
-
6.2.1.5 Alcanivorax borkumensis Q0VPF7 beta-subunit; gene sucCD
-
6.2.1.5 Alcanivorax borkumensis Q0VPF8 alpha-subunit; gene sucCD
-
6.2.1.5 Alcanivorax borkumensis SK2 Q0VPF7 beta-subunit; gene sucCD
-
6.2.1.5 Alcanivorax borkumensis SK2 Q0VPF8 alpha-subunit; gene sucCD
-
6.2.1.5 Escherichia coli K-12 P0A836 beta-subunit; gene sucCD
-
6.2.1.5 Escherichia coli K-12 P0AGE9 alpha-subunit; gene sucCD
-
6.2.1.5 Escherichia coli K-12 BL21 P0A836 beta-subunit; gene sucCD
-
6.2.1.5 Escherichia coli K-12 BL21 P0AGE9 alpha-subunit; gene sucCD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.5 recombinant C-terminally His-tagged enzyme 1.55fold from Escherichia coli strain BL21(DE3)-pLysS by nickel affinity chromatography Alcanivorax borkumensis
6.2.1.5 recombinant enzyme 1.07fold from Escherichia coli strain BL21(DE3)-pLysS by two different steps of anion exchange chromatography and affinity chromatography Advenella mimigardefordensis
6.2.1.5 recombinant enzyme 1.47fold from Escherichia coli strain BL21(DE3)-pLysS by anion exchange and affinity chromatography Escherichia coli K-12

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.5 1 2 purified recombinant enzyme, pH 7.4, 30°C, succinyl-CoA/ADP formation Advenella mimigardefordensis
6.2.1.5 4.14
-
 purified recombinant enzyme, pH 7.4, 30°C, succinyl-CoA/ADP formation Alcanivorax borkumensis
6.2.1.5 18.6
-
 purified recombinant enzyme, pH 7.4, 30°C, succinyl-CoA/ADP formation Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.5 ATP + 3-sulfinopropionate + CoA
-
 Escherichia coli K-12 ADP + phosphate + 3-sulfinopropionyl-CoA
-
 r
6.2.1.5 ATP + 3-sulfinopropionate + CoA
-
 Advenella mimigardefordensis ADP + phosphate + 3-sulfinopropionyl-CoA
-
 r
6.2.1.5 ATP + 3-sulfinopropionate + CoA
-
 Advenella mimigardefordensis DPN7 ADP + phosphate + 3-sulfinopropionyl-CoA
-
 r
6.2.1.5 ATP + 3-sulfinopropionate + CoA
-
 Escherichia coli K-12 BL21 ADP + phosphate + 3-sulfinopropionyl-CoA
-
 r
6.2.1.5 ATP + D-malate + CoA
-
 Escherichia coli K-12 ADP + phosphate + D-malyl-CoA
-
 r
6.2.1.5 ATP + D-malate + CoA
-
 Advenella mimigardefordensis ADP + phosphate + D-malyl-CoA
-
 r
6.2.1.5 ATP + D-malate + CoA
-
 Alcanivorax borkumensis ADP + phosphate + D-malyl-CoA
-
 r
6.2.1.5 ATP + D-malate + CoA
-
 Alcanivorax borkumensis SK2 ADP + phosphate + D-malyl-CoA
-
 r
6.2.1.5 ATP + D-malate + CoA
-
 Advenella mimigardefordensis DPN7 ADP + phosphate + D-malyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA
-
 Escherichia coli K-12 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA
-
 Advenella mimigardefordensis ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA
-
 Alcanivorax borkumensis ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA
-
 Alcanivorax borkumensis SK2 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA
-
 Advenella mimigardefordensis DPN7 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + itaconate + CoA
-
 Escherichia coli K-12 BL21 ADP + phosphate + itaconyl-CoA
-
 r
6.2.1.5 ATP + L-malate + CoA
-
 Escherichia coli K-12 ADP + phosphate + L-malyl-CoA
-
 r
6.2.1.5 ATP + L-malate + CoA
-
 Advenella mimigardefordensis ADP + phosphate + L-malyl-CoA
-
 r
6.2.1.5 ATP + L-malate + CoA
-
 Alcanivorax borkumensis ADP + phosphate + L-malyl-CoA
-
 r
6.2.1.5 ATP + L-malate + CoA
-
 Alcanivorax borkumensis SK2 ADP + phosphate + L-malyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA
-
 Advenella mimigardefordensis ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA
-
 Alcanivorax borkumensis ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA ATP is the preferred cosubstrate Escherichia coli K-12 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA
-
 Alcanivorax borkumensis SK2 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA
-
 Advenella mimigardefordensis DPN7 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 ATP + succinate + CoA ATP is the preferred cosubstrate Escherichia coli K-12 BL21 ADP + phosphate + succinyl-CoA
-
 r
6.2.1.5 GTP + succinate + CoA
-
 Escherichia coli K-12 GDP + phosphate + succinyl-CoA
-
 r
6.2.1.5 GTP + succinate + CoA
-
 Escherichia coli K-12 BL21 GDP + phosphate + succinyl-CoA
-
 r
6.2.1.5 additional information substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, 3-sulfinopropionate, fumarate, maleate, and 2,2'-thiodiacetate Advenella mimigardefordensis ?
-
 ?
6.2.1.5 additional information substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate Alcanivorax borkumensis ?
-
 ?
6.2.1.5 additional information the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4, substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate Escherichia coli K-12 ?
-
 ?
6.2.1.5 additional information the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4. substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate Escherichia coli K-12 ?
-
 ?
6.2.1.5 additional information substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate Alcanivorax borkumensis SK2 ?
-
 ?
6.2.1.5 additional information substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, 3-sulfinopropionate, fumarate, maleate, and 2,2'-thiodiacetate Advenella mimigardefordensis DPN7 ?
-
 ?
6.2.1.5 additional information the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4. substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate Escherichia coli K-12 BL21 ?
-
 ?
6.2.1.5 additional information the enzyme is able to use ATP as well as GTP as a cosubstrate, cf. EC 6.2.1.4, substrate specificity, overview. Besides the preference for the physiological substrates succinate, itaconate, ATP, and CoA, high enzyme activity is additionally determined for both enantiomeric forms of malate, amounting to 10-21% of the activity with succinate, strong resemblance of SucCD to L-malate-CoA ligase, EC 6.2.1.9. No activity with sulfosuccinate, mercaptosuccinate, tartrate, acetate, butyrate, propionate, levulinate, valerate, malonate, glutarate, adipate, fumarate, maleate, and 2,2'-thiodiacetate Escherichia coli K-12 BL21 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.5 SucCD
-
 Escherichia coli K-12
6.2.1.5 SucCD
-
 Advenella mimigardefordensis
6.2.1.5 SucCD
-
 Alcanivorax borkumensis
6.2.1.5 succinate coenzyme A ligase
-
 Escherichia coli K-12
6.2.1.5 succinate coenzyme A ligase
-
 Advenella mimigardefordensis
6.2.1.5 succinate coenzyme A ligase
-
 Alcanivorax borkumensis
6.2.1.5 succinate-CoA ligase
-
 Escherichia coli K-12
6.2.1.5 succinate-CoA ligase
-
 Advenella mimigardefordensis
6.2.1.5 succinate-CoA ligase
-
 Alcanivorax borkumensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.5 30
-
 assay at Escherichia coli K-12
6.2.1.5 30
-
 assay at Advenella mimigardefordensis
6.2.1.5 30
-
 assay at Alcanivorax borkumensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.5 0.2
-
 3-sulfinopropionate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.2
-
 3-sulfinopropionate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 0.5
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 1.1
-
 L-malate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 1.2
-
 D-malate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 1.5
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 1.6
-
 CoA recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 1.7
-
 D-malate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 1.8
-
 L-malate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 2.1
-
 D-malate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 2.6
-
 L-malate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 2.7
-
 succinate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 3.6
-
 ATP recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 5.3
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 14.3
-
 succinate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 19.6
-
 ATP recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 26.7
-
 CoA recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 31.1
-
 succinate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 40.3
-
 CoA recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 58.8
-
 ATP recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.5 7.4
-
 assay at Escherichia coli K-12
6.2.1.5 7.4
-
 assay at Advenella mimigardefordensis
6.2.1.5 7.4
-
 assay at Alcanivorax borkumensis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.5 ADP
-
 Escherichia coli K-12
6.2.1.5 ADP
-
 Advenella mimigardefordensis
6.2.1.5 ADP
-
 Alcanivorax borkumensis
6.2.1.5 ATP
-
 Escherichia coli K-12
6.2.1.5 ATP
-
 Advenella mimigardefordensis
6.2.1.5 ATP
-
 Alcanivorax borkumensis
6.2.1.5 GDP
-
 Escherichia coli K-12
6.2.1.5 GTP
-
 Escherichia coli K-12

General Information

EC Number General Information Comment Organism
6.2.1.5 evolution malate-CoA ligases and succinate-CoA ligases might have the same evolutionary origin Escherichia coli K-12

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.5 0.1
-
 3-sulfinopropionate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.3
-
 L-malate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 0.3
-
 D-malate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.4
-
 D-malate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 0.6
-
 D-malate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 0.7
-
 L-malate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 0.8
-
 L-malate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 1
-
 3-sulfinopropionate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 3.1
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 3.3
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 14.8
-
 ATP recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 15.1
-
 Itaconate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 17.2
-
 succinate recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 101.4
-
 succinate recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 171.1
-
 succinate recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 292.5
-
 ATP recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis
6.2.1.5 354.4
-
 ATP recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 372.3
-
 CoA recombinant enzyme, pH 7.4, 30°C Alcanivorax borkumensis
6.2.1.5 461.3
-
 CoA recombinant enzyme, pH 7.4, 30°C Escherichia coli K-12
6.2.1.5 1100
-
 CoA recombinant enzyme, pH 7.4, 30°C Advenella mimigardefordensis