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Literature summary extracted from

  • Hou, J.; Feng, B.; Han, J.; Liu, H.; Zhao, D.; Zhou, J.; Xiang, H.
    Haloarchaeal-type beta-ketothiolases involved in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in Haloferax mediterranei (2013), Appl. Environ. Microbiol., 79, 5104-5111.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.9 synthesis the enzyme has biotechnological potential in haloarchaea for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with controllable 3-oxopentano content, from unrelated cheap carbon sources Haloferax mediterranei

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.9 additional information the deletion mutant strain expressing the enzyme with an S83A, H281Q, or H331Q mutation in BktBalpha does not accumulate polyhydroxyalkanoate at all, whereas strains expressing the enzyme with a C365A or C365S mutation in subunit BktBalpha have no significant effect on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation Haloferax mediterranei
2.3.1.9 additional information the introduction of the S84A, H282Q, or H332Q mutation into subunit PhaAalpha results in inactive enzyme, while the C366A or C366S mutation does not obviously affect PhaA activity Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 2 acetyl-CoA Haloferax mediterranei the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) CoA + acetoacetyl-CoA
-
 ?
2.3.1.9 2 acetyl-CoA Haloferax mediterranei DSM 1411 the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) CoA + acetoacetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Haloferax mediterranei I3R3D1 and I3R3D0 I3R3D1: catalytic subunit PhaAalpha, I3RA71: subunit PhaAbeta
-
2.3.1.9 Haloferax mediterranei I3RA72 and I3RA71 I3RA72: catalytic subunit BktBalpha, I3RA71: subunit BktBbeta
-
2.3.1.9 Haloferax mediterranei DSM 1411 I3R3D1 and I3R3D0 I3R3D1: catalytic subunit PhaAalpha, I3RA71: subunit PhaAbeta
-
2.3.1.9 Haloferax mediterranei DSM 1411 I3RA72 and I3RA71 I3RA72: catalytic subunit BktBalpha, I3RA71: subunit BktBbeta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 2 acetyl-CoA
-
 Haloferax mediterranei CoA + acetoacetyl-CoA
-
 ?
2.3.1.9 2 acetyl-CoA the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Haloferax mediterranei CoA + acetoacetyl-CoA
-
 ?
2.3.1.9 2 acetyl-CoA
-
 Haloferax mediterranei DSM 1411 CoA + acetoacetyl-CoA
-
 ?
2.3.1.9 2 acetyl-CoA the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Haloferax mediterranei DSM 1411 CoA + acetoacetyl-CoA
-
 ?
2.3.1.9 acetyl-CoA + propionyl-CoA
-
 Haloferax mediterranei CoA + 3-oxopentanoyl-CoA
-
 ?
2.3.1.9 acetyl-CoA + propionyl-CoA
-
 Haloferax mediterranei DSM 1411 CoA + 3-oxopentanoyl-CoA
-
 ?
2.3.1.9 additional information the catalytic triad of BktBalpha is likely to be Ser-His-His Haloferax mediterranei ?
-
 ?
2.3.1.9 additional information the catalytic triad of PhaAalpha is likely to be Ser-His-His Haloferax mediterranei ?
-
 ?
2.3.1.9 additional information the catalytic triad of PhaAalpha is likely to be Ser-His-His Haloferax mediterranei DSM 1411 ?
-
 ?
2.3.1.9 additional information the catalytic triad of BktBalpha is likely to be Ser-His-His Haloferax mediterranei DSM 1411 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 ? composed of two different types of subunits: catalytic subunit PhaAalpha, and subunit PhaABbeta Haloferax mediterranei
2.3.1.9 More composed of two different types of subunits: catalytic subunit BktBalpha, and BktBbeta Haloferax mediterranei

Synonyms

EC Number Synonyms Comment Organism
2.3.1.9 HFX_1022 locus name, subunit PhaAbeta Haloferax mediterranei
2.3.1.9 HFX_1023 locus name, subunit PhaAalpha Haloferax mediterranei
2.3.1.9 HFX_6003 locus name, subunit BktBbeta Haloferax mediterranei
2.3.1.9 HFX_6004 locus name, subunit BktBalpha Haloferax mediterranei

General Information

EC Number General Information Comment Organism
2.3.1.9 physiological function the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Haloferax mediterranei