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Literature summary extracted from

  • Liu, D.F.; Ding, H.T.; Du, Y.Q.; Zhao, Y.H.; Jia, X.M.
    Cloning, expression, and characterization of a wide-pH-range stable phosphite dehydrogenase from Pseudomonas sp. K in Escherichia coli (2012), Appl. Biochem. Biotechnol., 166, 1301-1313.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.1.1 expression in Escherichia coli Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.20.1.1 analysis of the electrostatic potentials of the interfaces between the subunits. Regions A, B, and C are almost electroneutral Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.20.1.1 Cd2+ 1 mM, no residual activity Pseudomonas sp.
1.20.1.1 Cu2+ 1 mM, about 55% of initial activity Pseudomonas sp.
1.20.1.1 Mn2+ 1 mM, about 65% of initial activity Pseudomonas sp.
1.20.1.1 Zn2+ 1 mM, about 35% of initial activity Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.1.1 0.022
-
 NAD+ pH 7.0, 25°C Pseudomonas sp.
1.20.1.1 0.475
-
 phosphite pH 7.0, 25°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.20.1.1 EDTA 1 mM, about 155% of initial activity Pseudomonas sp.
1.20.1.1 Fe3+ 1 mM, about 150% of initial activity Pseudomonas sp.
1.20.1.1 Mg2+ 1 mM, about 125% of initial activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.1.1 35000
-
 2 * 35000, SDS-PAGE Pseudomonas sp.
1.20.1.1 70000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.20.1.1 Pseudomonas sp. F2YGD2
-
-
1.20.1.1 Pseudomonas sp. K F2YGD2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.1.1 recombinant protein Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.20.1.1 3.49
-
 pH 7.0, 25°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.1.1 phosphite + H2O + NAD+
-
 Pseudomonas sp. phosphate + NADH + H+
-
 ?
1.20.1.1 phosphite + H2O + NAD+
-
 Pseudomonas sp. K phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.1.1 dimer 2 * 35000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.1.1 45
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.20.1.1 20
-
 about 13% of maximum activity Pseudomonas sp.
1.20.1.1 55
-
 less than 10% of maximum activity Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.20.1.1 40
-
 stable below Pseudomonas sp.
1.20.1.1 45
-
 hlf-life 35 min Pseudomonas sp.
1.20.1.1 55
-
 complete inactivation Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.1.1 3
-
 NaH2PO4/H3PO4 buffer Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.20.1.1 2.5 10.5
-
 Pseudomonas sp.
1.20.1.1 7
-
 assay at, activity at pH 3.0 is about 2.2fold higher than at pH 7.0 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.20.1.1 5 10.5 1 h, more than 80% residual activity Pseudomonas sp.