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Literature summary extracted from
- Changing the regioselectivity of the tryptophan 7-halogenase PrnA by site-directed mutagenesis (2011), Angew. Chem. Int. Ed. Engl., 50, 2951-2953.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.9 | expressed in Escherichia coli | Pseudomonas fluorescens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.19.9 | F103A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
| 1.14.19.9 | F103A/W455A | inactive | Pseudomonas fluorescens |
| 1.14.19.9 | H101A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
| 1.14.19.9 | W455A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.9 | 0.0499 | - |
tryptophan | His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas fluorescens |  |
| 1.14.19.9 | 0.1986 | - |
tryptophan | His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
| 1.14.19.9 | 1.785 | - |
tryptophan | His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
| 1.14.19.9 | 1.814 | - |
tryptophan | His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.9 | tryptophan + FADH2 + Cl- + O2 + H+ | Pseudomonas fluorescens | - |
7-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.9 | Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.9 | nickel chelating Sepharose column chromatography | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.9 | tryptophan + FADH2 + Cl- + O2 + H+ | - |
Pseudomonas fluorescens | 7-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.9 | PrnA | - |
Pseudomonas fluorescens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.9 | 0.018 | - |
tryptophan | His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
| 1.14.19.9 | 0.021 | - |
tryptophan | His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
| 1.14.19.9 | 0.067 | - |
tryptophan | His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
| 1.14.19.9 | 0.11 | - |
tryptophan | His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.9 | flavin | dependent on | Pseudomonas fluorescens | |
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