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Literature summary extracted from

  • Wang, C.; Fan, X.; Cao, X.; Liu, X.; Li, L.; Su, X.
    Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans (2012), Acta Crystallogr. Sect. F, 68, 565-568.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expressed in Escherichia coli BL21(DE3) cells Streptococcus mutans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.6 sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 0.1 M NaCl, 2.0 M ammonium sulfate Streptococcus mutans
5.3.1.6 sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M Tris-HCl pH 8.5 and 25% (w/v) PEG 3350, 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.5 Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 23400
-
x * 23400, isoform RpiB, calculated from amino acid sequence Streptococcus mutans
5.3.1.6 24570
-
x * 24570, isoform RpiA, calculated from amino acid sequence Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate Streptococcus mutans
-
D-ribulose 5-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Streptococcus mutans Q8DRS5
-
-
5.3.1.6 Streptococcus mutans Q8DTT9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 Ni2+-chelating column chromatography and Superdex S75 gel filtration Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate
-
Streptococcus mutans D-ribulose 5-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 ? x * 23400, isoform RpiB, calculated from amino acid sequence Streptococcus mutans
5.3.1.6 ? x * 24570, isoform RpiA, calculated from amino acid sequence Streptococcus mutans

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 RpiA isoform Streptococcus mutans
5.3.1.6 RpiB isoform Streptococcus mutans
5.3.1.6 SMU1234 isoform Streptococcus mutans
5.3.1.6 SMU2142 isoform Streptococcus mutans