EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.33 | expression in Escherichia coli BL21(DE3) Star cells | Methanocaldococcus jannaschii |
4.2.1.36 | expressed in Escherichia coli BL21(DE3) Star cells | Methanocaldococcus jannaschii |
4.2.1.114 | expression in Escherichia coli BL21(DE3) Star cells | Methanocaldococcus jannaschii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.1.33 | sitting-drop vapour-diffusion methodand hanging-drop vapour diffusion at 20°C, structures of oxidized and reduced forms of the large subunit of isopropylmalate isomerase (ox-MJ0499 and red-MJ0499, respectively) are reported at 1.8 and 2.7 A resolution, respectively. Significant large conformational changes are observed in the active site of red-MJ0499 when compared with ox-MJ0499 | Methanocaldococcus jannaschii |
4.2.1.36 | sitting drop vapor diffusion method, small subunit, using 60% (w/v) 2-methyl-2,4-pentanediol and 0.1 M bicine pH 9.7 | Methanocaldococcus jannaschii |
4.2.1.114 | sitting-drop vapour-diffusion method and hanging-drop vapour diffusion at 20°C, structures of the large subunit of homoaconise is reported at 2.5 A resolution | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.33 | Iron-sulfur cluster | the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site | Methanocaldococcus jannaschii | |
4.2.1.114 | Iron-sulfur cluster | the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.33 | Methanocaldococcus jannaschii | P81291 | large subunit of 3-isopropylmalate dehydratase | - |
4.2.1.33 | Methanocaldococcus jannaschii DSM 2661 | P81291 | large subunit of 3-isopropylmalate dehydratase | - |
4.2.1.36 | Methanocaldococcus jannaschii | P81291 | - |
- |
4.2.1.114 | Methanocaldococcus jannaschii | Q58409 | large subunit of homoaconitase | - |
4.2.1.114 | Methanocaldococcus jannaschii DSM 2661 | Q58409 | large subunit of homoaconitase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.33 | - |
Methanocaldococcus jannaschii |
4.2.1.36 | HisTrap HP column chromatography and Superdex 200 gel filtration | Methanocaldococcus jannaschii |
4.2.1.114 | - |
Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.36 | cis-homoaconitate | - |
Methanocaldococcus jannaschii | homoisocitrate + H2O | - |
? | |
4.2.1.36 | cis-homoaconitate | - |
Methanocaldococcus jannaschii DSM 2661 | homoisocitrate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.36 | dimer | x-ray crystallography | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.33 | isopropylmalate isomerase | - |
Methanocaldococcus jannaschii |
4.2.1.33 | leuC | - |
Methanocaldococcus jannaschii |
4.2.1.33 | MJ0499 | gene name, large subunit of 3-isopropylmalate dehydratase | Methanocaldococcus jannaschii |
4.2.1.36 | Homoaconitase | - |
Methanocaldococcus jannaschii |
4.2.1.36 | leuC | large subunit | Methanocaldococcus jannaschii |
4.2.1.36 | MJ0499 | - |
Methanocaldococcus jannaschii |
4.2.1.114 | HacA | - |
Methanocaldococcus jannaschii |
4.2.1.114 | Homoaconitase | - |
Methanocaldococcus jannaschii |
4.2.1.114 | MJ1003 | gene name, large subunit of homoaconitase | Methanocaldococcus jannaschii |