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Literature summary extracted from

  • Scaletti, E.R.; Luckner, S.R.; Krause, K.L.
    Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50) (2012), Acta Crystallogr. Sect. D, 68, 82-92.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 enzyme expression in Escherichia coli strain BL21(DE3)/pMB1978 Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.1 purified recombinant enzyme from strain Mu50, sitting drop method, mixing of 12 mg/ml protein in 20 mM Tris-HCl, pH 7.6, with 100 mM sodium acetate trihydrate, pH 5.0, and 1.8 M ammonium sulfate, 7 days, X-ray diffraction structure determination and analysis at 2.15 A resolution, molecular replacement Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 additional information the active-site binding pocket, dimer interface and active-site entryway of the enzyme are potential targets for structure-aided inhibitor design, formation of a template for structure-based drug-development efforts targeting the enzyme, overview Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 0.89
-
D-alanine pH not specified in the publication, 30°C, recombinant enzyme Staphylococcus aureus
5.1.1.1 2.77
-
L-alanine pH not specified in the publication, 30°C, recombinant enzyme Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 42800
-
2 * 42800, recombinant enzyme, SDS-PAGE Staphylococcus aureus
5.1.1.1 73000
-
recombinant enzyme, dynamic light scattering Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.1 L-alanine Staphylococcus aureus
-
D-alanine
-
r
5.1.1.1 L-alanine Staphylococcus aureus Mu50
-
D-alanine
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Staphylococcus aureus P63479
-
-
5.1.1.1 Staphylococcus aureus Mu50 P63479
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1 recombinant enzyme from Escherichia coli strain BL21(DE3)/pMB1978 by anion exchange and hydrophobic interaction chromatography, followed by gel filtration and dialysis to over 95% purity Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-alanine
-
Staphylococcus aureus D-alanine
-
r
5.1.1.1 L-alanine
-
Staphylococcus aureus Mu50 D-alanine
-
r

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 42800, recombinant enzyme, SDS-PAGE Staphylococcus aureus
5.1.1.1 More dimer interfaces for the enzyme is 2510 A2 Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
5.1.1.1 ALR
-
Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 30
-
assay at Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate dependent on Staphylococcus aureus

General Information

EC Number General Information Comment Organism
5.1.1.1 malfunction because D-alanine is an essential component of the bacterial cell-wall peptidoglycan, inhibition of alanine racemase is lethal to prokaryotes Staphylococcus aureus