EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.1.1 | enzyme expression in Escherichia coli strain BL21(DE3)/pMB1978 | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.1.1.1 | purified recombinant enzyme from strain Mu50, sitting drop method, mixing of 12 mg/ml protein in 20 mM Tris-HCl, pH 7.6, with 100 mM sodium acetate trihydrate, pH 5.0, and 1.8 M ammonium sulfate, 7 days, X-ray diffraction structure determination and analysis at 2.15 A resolution, molecular replacement | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.1 | additional information | the active-site binding pocket, dimer interface and active-site entryway of the enzyme are potential targets for structure-aided inhibitor design, formation of a template for structure-based drug-development efforts targeting the enzyme, overview | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.1 | 0.89 | - |
D-alanine | pH not specified in the publication, 30°C, recombinant enzyme | Staphylococcus aureus | |
5.1.1.1 | 2.77 | - |
L-alanine | pH not specified in the publication, 30°C, recombinant enzyme | Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.1.1 | 42800 | - |
2 * 42800, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
5.1.1.1 | 73000 | - |
recombinant enzyme, dynamic light scattering | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.1 | L-alanine | Staphylococcus aureus | - |
D-alanine | - |
r | |
5.1.1.1 | L-alanine | Staphylococcus aureus Mu50 | - |
D-alanine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.1 | Staphylococcus aureus | P63479 | - |
- |
5.1.1.1 | Staphylococcus aureus Mu50 | P63479 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.1.1 | recombinant enzyme from Escherichia coli strain BL21(DE3)/pMB1978 by anion exchange and hydrophobic interaction chromatography, followed by gel filtration and dialysis to over 95% purity | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.1 | L-alanine | - |
Staphylococcus aureus | D-alanine | - |
r | |
5.1.1.1 | L-alanine | - |
Staphylococcus aureus Mu50 | D-alanine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.1.1 | dimer | 2 * 42800, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
5.1.1.1 | More | dimer interfaces for the enzyme is 2510 A2 | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.1.1 | ALR | - |
Staphylococcus aureus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.1.1 | 30 | - |
assay at | Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.1 | pyridoxal 5'-phosphate | dependent on | Staphylococcus aureus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.1.1.1 | malfunction | because D-alanine is an essential component of the bacterial cell-wall peptidoglycan, inhibition of alanine racemase is lethal to prokaryotes | Staphylococcus aureus |