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Literature summary extracted from
- Evaluation of bioreductive activation of anticancer drugs idarubicin and mitomycin C by NADH-cytochrome b5 reductase and cytochrome P450 2B4 (2013), Xenobiotica, 43, 263-275.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.2.2 | microsome | - |
Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.2 | 2 ferricytochrome b5 + NADH | Bos taurus | - |
2 ferrocytochrome b5 + NAD+ + H+ | - |
? |  |
| 1.6.2.2 | additional information | Bos taurus | poor activity with the anticancer drug mitomycin C, no activity with anticancer drug idarubicin. The quinone antitumor agents are used in the treatment of several human neoplasms | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | native enzyme from liver by anion-exchange chromatography of the detergent solubilized microsomes on two successive DEAE-cellulose columns and an affinity chromatography on adenosine 5'-diphosphate-agarose, to homogeneity | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.2 | 2 ferricytochrome b5 + NADH | - |
Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 1.6.2.2 | additional information | poor activity with the anticancer drug mitomycin C, no activity with anticancer drug idarubicin. The quinone antitumor agents are used in the treatment of several human neoplasms | Bos taurus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.2.2 | NADH-cytochrome b5 reductase | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 25 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 6.6 | 7.5 | assay at | Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 6.6 | 7.5 | enzyme activity is higher at pH 7.5 compared to pH 6.6 | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.2 | NADH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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