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Literature summary extracted from

  • Faucher, F.; Duclos, S.; Bandaru, V.; Wallace, S.S.; Doublie, S.
    Crystal structures of two archaeal 8-oxoguanine DNA glycosylases provide structural insight into guanine/8-oxoguanine distinction (2009), Structure, 17, 703-712.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.B5
-
 Methanocaldococcus jannaschii
3.2.2.B5
-
 Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.B5 hanging-drop vapor diffusion at 12°C Saccharolobus solfataricus
3.2.2.B5 hanging-drop vapor diffusion at 12°C. A structural comparison with 8-oxoguanine DNA glycosylase 1 and archaeal GO glycosylase suggests that the C-terminal lysine of 8-oxoguanine DNA glycosylase 2 may play a key role in discriminating between guanine and 8-oxoguanine Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.B5 K128Q glycosylase-deficient mutant Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.B5 DNA containing 8-oxoguanine + H2O Methanocaldococcus jannaschii base excision repair pathway DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O Methanocaldococcus jannaschii DSM 2661 base excision repair pathway DNA with an abasic site + 8-oxoguanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.B5 Methanocaldococcus jannaschii Q58134
-
-
3.2.2.B5 Methanocaldococcus jannaschii DSM 2661 Q58134
-
-
3.2.2.B5 Saccharolobus solfataricus Q97ZK2
-
-
3.2.2.B5 Saccharolobus solfataricus P2 Q97ZK2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.B5
-
 Methanocaldococcus jannaschii
3.2.2.B5
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.B5 DNA containing 8-oxoguanine + H2O base excision repair pathway Methanocaldococcus jannaschii DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O DNA substrate used for glycosylase assay: 5'-TGTCAATAGCAAG-X-GGAGAAGTCAATCGTAGTCT-3' for the damage-containing strand, where X is either 8-oxoguanine or uracil, and 5'-AGACTCACGATTGACTTCTCC-Y-CTTGCTATTGACA-3' for the complementary strand, where Y is either one of the four bases. The enzyme displays little preference for the base opposite 8-oxoguanine Methanocaldococcus jannaschii DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O base excision repair pathway Methanocaldococcus jannaschii DSM 2661 DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O DNA substrate used for glycosylase assay: 5'-TGTCAATAGCAAG-X-GGAGAAGTCAATCGTAGTCT-3' for the damage-containing strand, where X is either 8-oxoguanine or uracil, and 5'-AGACTCACGATTGACTTCTCC-Y-CTTGCTATTGACA-3' for the complementary strand, where Y is either one of the four bases. The enzyme displays little preference for the base opposite 8-oxoguanine Methanocaldococcus jannaschii DSM 2661 DNA with an abasic site + 8-oxoguanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.B5 8-oxoguanine DNA glycosylase bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Saccharolobus solfataricus
3.2.2.B5 MjaOgg2 bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Methanocaldococcus jannaschii
3.2.2.B5 Ogg2
-
 Saccharolobus solfataricus
3.2.2.B5 SSO0904 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.B5 50
-
 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.B5 8.5
-
 assay at Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
3.2.2.B5 physiological function base excision repair pathway Methanocaldococcus jannaschii