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Literature summary extracted from

  • Chen, M.; Drury, J.E.; Penning, T.M.
    Substrate specificity and inhibitor analyses of human steroid 5beta-reductase (AKR1D1) (2011), Steroids, 76, 484-490.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.3 4-benzoylbenzoic acid
-
 Homo sapiens
1.3.1.3 chenodeoxycholic acid
-
 Homo sapiens
1.3.1.3 indomethacin
-
 Homo sapiens
1.3.1.3 Mefenamic acid
-
 Homo sapiens
1.3.1.3 additional information substrate inhibition is observed with C18 to C21 steroids provided that the C11 position is unsubstituted Homo sapiens
1.3.1.3 Ursodeoxycholic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.0003
-
 cholestenone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0008
-
 4-cholesten-7alpha-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0009
-
 4-androstene-3,17-dione pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0022
-
 corticosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0025
-
 aldosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0027
-
 testosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0029
-
 Epitestosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.003
-
 4-estren-17beta-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0032
-
 1,4-androstadien-17beta-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0131
-
 cortisol pH 6.0, 37°C Homo sapiens
1.3.1.3 0.0151
-
 cortisone pH 6.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 1,4-androstadien-17beta-ol-3-one + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 4-androstene-3,17-dione + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 4-cholesten-7alpha-ol-3-one + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 4-estren-17beta-ol-3-one + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 aldosterone + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 cholestenone + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 corticosterone + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 cortisol + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 cortisone + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 epitestosterone + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.3.1.3 additional information substrate specificity of homogeneous human recombinant AKR1D1 using C18, C19, C21, and C27 DELTA4-ketosteroids, AKR1D1 proficiently reduces all the steroids tested at physiological pH, overview Homo sapiens ?
-
 ?
1.3.1.3 testosterone + NADPH + H+
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 AKR1D1
-
 Homo sapiens
1.3.1.3 aldo-keto reductase 1D1
-
 Homo sapiens
1.3.1.3 steroid 5beta-reductase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.3 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.3 0.01
-
 cholestenone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.032
-
 corticosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.033
-
 4-cholesten-7alpha-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 0.035
-
 1,4-androstadien-17beta-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 0.045
-
 cortisol pH 6.0, 37°C Homo sapiens
1.3.1.3 0.045
-
 4-estren-17beta-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 0.1
-
 4-androstene-3,17-dione pH 6.0, 37°C Homo sapiens
1.3.1.3 0.1
-
 Epitestosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.14
-
 testosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.15
-
 aldosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 0.195
-
 cortisone pH 6.0, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.3 6
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.3.1.3 additional information existence of a small alternative substrate binding pocket, structure-activity relationship, overview Homo sapiens
1.3.1.3 physiological function human steroid 5beta-reductase, i.e. aldo-keto reductase 1D1, catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of DELTA4-ketosteroids to yield an A/B cis-ring junction. This cis configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. AKR1D1 is the only enzyme necessary for all the 5-steroid metabolites present in humans Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.3 2.77
-
 4-androstene-3,17-dione pH 6.0, 37°C Homo sapiens
1.3.1.3 3.44
-
 cortisol pH 6.0, 37°C Homo sapiens
1.3.1.3 10.94
-
 1,4-androstadien-17beta-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 12.91
-
 cortisone pH 6.0, 37°C Homo sapiens
1.3.1.3 14.54
-
 corticosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 15
-
 4-estren-17beta-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 33.33
-
 cholestenone pH 6.0, 37°C Homo sapiens
1.3.1.3 34.48
-
 Epitestosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 41.25
-
 4-cholesten-7alpha-ol-3-one pH 6.0, 37°C Homo sapiens
1.3.1.3 51.85
-
 testosterone pH 6.0, 37°C Homo sapiens
1.3.1.3 60
-
 aldosterone pH 6.0, 37°C Homo sapiens