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Literature summary extracted from

  • Da Silva, A.; Jacques, R.; Andreazza, R.; Bento, F.; Camargo, F.
    The effects of trace elements, cations, and environmental conditions on protocatechuate 3,4-dioxygenase activity (2013), Sci. Agric. (Piracicaba, Braz.), 70, 68-73.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.3 phylogenetic tree Leifsonia sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.3 additional information immobilization of the enzyme, the immobilized extract exhibited higher enzyme activity than the cell-free extract in the presence of trace elements and cations Leifsonia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.3 Cu2+ increases enzyme activity for both cell-free and immobilized extracts by 8% and 44%, respectively Leifsonia sp.
1.13.11.3 Fe3+ increases enzyme activity for both cell-free and immobilized extracts by 16% and 99%, respectively Leifsonia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2 Leifsonia sp.
-
 3-carboxy-cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Leifsonia sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.3 culture condition:phenanthrene-grown cell
-
 Leifsonia sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Leifsonia sp. 3-carboxy-cis,cis-muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.3 P3,4O enzyme
-
 Leifsonia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.3 35
-
 optimum for the cell-free enzyme extract Leifsonia sp.
1.13.11.3 55
-
 optimum for the immobilized enzyme extract Leifsonia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 7.5
-
 assay at Leifsonia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.3 4 9 activity range, profile overview Leifsonia sp.
1.13.11.3 6.5 8 80% of maximal activity within this range Leifsonia sp.