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Literature summary extracted from

  • Chen, S.C.; Wu, P.H.; Su, Y.C.; Wen, T.N.; Wei, Y.S.; Wang, N.C.; Hsu, C.A.; Wang, A.H.; Shyur, L.F.
    Biochemical characterization of a novel laccase from the basidiomycete fungus Cerrena sp. WR1 (2012), Protein Eng. Des. Sel., 25, 761-769.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 gene lcc3, DNA and amino acid sequence determination and analysis, phylogenetic analysis Cerrena sp. WR1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0033
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 50°C Cerrena sp. WR1
1.10.3.2 0.393
-
 2,6-dimethoxyphenol pH 3.0, 50°C Cerrena sp. WR1
1.10.3.2 0.849
-
 guaiacol pH 3.0, 50°C Cerrena sp. WR1
1.10.3.2 0.881
-
 catechol pH 3.0, 50°C Cerrena sp. WR1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular the enzyme is secreted Cerrena sp. WR1
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 57500
-
 x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE Cerrena sp. WR1
1.10.3.2 64100
-
 x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE Cerrena sp. WR1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 4 catechol + O2 Cerrena sp. WR1
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 acetonitril 77.5% activity remaining after 14 days in 25% Cerrena sp. WR1
1.10.3.2 dimethyl sulfoxide 55.8% activity remaining after 14 days in 25% Cerrena sp. WR1
1.10.3.2 Ethanol 84.8% activity remaining after 14 days in 25% Cerrena sp. WR1
1.10.3.2 Methanol 74.6% activity remaining after 14 days in 25% Cerrena sp. WR1
1.10.3.2 N,N-dimethylformamide 81.2% activity remaining after 14 days in 25% Cerrena sp. WR1

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Cerrena sp. WR1 E7BLR0 gene lcc3
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein laccase Lcc3 is N-linked glycosylated, deglycosylation by peptide N-glycosidase F, the enzyme contains six potential N-glycosylation sites, i.e. N35, N236, N272, N311, N453 and N489, with a conserved sequence of NxS/T, ire found in the deduced Lcc3 amino acid sequence Cerrena sp. WR1

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 24.9fold from strain WR1, by two steps of anion exchange chromatography to over 96% homogeneity Cerrena sp. WR1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 144.3
-
 specific activity of the crude laccase broth, pH 3.0, 50°C Cerrena sp. WR1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2 2,6-dimethoxyphenol + O2
-
 Cerrena sp. WR1 ?
-
 ?
1.10.3.2 4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Cerrena sp. WR1 ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Cerrena sp. WR1 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 guaiacol + O2
-
 Cerrena sp. WR1 ?
-
 ?
1.10.3.2 additional information the enzyme efficiently performs dye decoloration of anthraquinone dye RBBR Cerrena sp. WR1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE Cerrena sp. WR1

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Lcc3
-
 Cerrena sp. WR1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Cerrena sp. WR1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 Tm of Lcc3 is determined at 73.9°C and it shows a t1/2 of 120 min at 50°C Cerrena sp. WR1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 109.2
-
 2,6-dimethoxyphenol pH 3.0, 50°C Cerrena sp. WR1
1.10.3.2 115.5
-
 catechol pH 3.0, 50°C Cerrena sp. WR1
1.10.3.2 147.9
-
 guaiacol pH 3.0, 50°C Cerrena sp. WR1
1.10.3.2 934.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 50°C Cerrena sp. WR1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena sp. WR1
1.10.3.2 3.5
-
 with substrate 2,6-dimethoxyphenol Cerrena sp. WR1
1.10.3.2 4.5
-
 with substrate guaiacol Cerrena sp. WR1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3
-
 loss of 50% activity after 43 h, 25°C Cerrena sp. WR1
1.10.3.2 4
-
 loss of 50% activity after 82 h, 25°C Cerrena sp. WR1
1.10.3.2 5
-
 loss of 20% activity after 100 h, 25°C Cerrena sp. WR1
1.10.3.2 6 10 no loss of activity after 100 h, 25°C Cerrena sp. WR1

General Information

EC Number General Information Comment Organism
1.10.3.2 evolution the enzyme belongs to the blue multicopper oxidase superfamily Cerrena sp. WR1