EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.3.1 | expression of recombinant AtraAOX2 with His6-tag at the C-terminal end in a baculovirus expression system | Amyelois transitella |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.1 | 2,4-dinitrophenol | 50 microM, 41.7% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | 4-hydroxymercuribenzoate | 50 microM, 55.3% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | antimycin A | 10 microM, 45.6% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | beta-ecdysone | 50 microM, 30.1% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | beta-estradiol | 50 microM, 60.2% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | Digitonin | 125 microM, 69.9% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | ethanol | 2% (v/v), 62.1% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | Isopropanol | 2% (v/v), 67.9% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | menadione | 100 microM, 63.1% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | methanol | 2% (v/v), 33.9% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | additional information | hydroquinone and sodium deoxycholate have no inhibitory effect | Amyelois transitella | |
1.2.3.1 | potassium cyanide | 1 mM, 71.8% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | Quinacrine | 1 mM, 34.9% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | rotenone | 10 microM, 89.3% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | Sodium azide | 5 mM, 52.4% residual AtraAOX2 activity | Amyelois transitella | |
1.2.3.1 | Triton X-100 | 0.01% (v/v), 71.8% residual AtraAOX2 activity | Amyelois transitella |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.3.1 | 140000 | - |
SDS-PAGE, AtraAOX2 monomer still shows strong activity | Amyelois transitella |
1.2.3.1 | 280000 | - |
Western blot analysis | Amyelois transitella |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.1 | Amyelois transitella | S5FPI8 | navel orangeworm | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.3.1 | by Ni-NTA column chromatography | Amyelois transitella |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.3.1 | antenna | male and female | Amyelois transitella | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.1 | (2E)-hex-2-enal + H2O + O2 | - |
Amyelois transitella | (2E)-hex-2-enoic acid + H2O2 | - |
? | |
1.2.3.1 | (2E)-non-2-enal + H2O + O2 | - |
Amyelois transitella | (2E)-non-2-enoic acid + H2O2 | - |
? | |
1.2.3.1 | acetaldehyde + H2O + O2 | - |
Amyelois transitella | acetic acid + H2O2 | - |
? | |
1.2.3.1 | acrolein + H2O + O2 | results indicate that AtraAOX2 also functions as a xenobiotic-degrading enzyme | Amyelois transitella | ? | - |
? | |
1.2.3.1 | benzaldehyde + H2O + O2 | - |
Amyelois transitella | benzoic acid + H2O2 | - |
? | |
1.2.3.1 | citral + H2O + O2 | low AtraAOX2 activity | Amyelois transitella | (2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2 | - |
? | |
1.2.3.1 | heptanal + H2O + O2 | - |
Amyelois transitella | heptanoic acid + H2O2 | - |
? | |
1.2.3.1 | hexanal + H2O + O2 | - |
Amyelois transitella | hexanoic acid + H2O2 | - |
? | |
1.2.3.1 | additional information | AtraAOX2 shows strong activity on aldehyde substrates derived from plants, but weak activity on Z11Z1316Ald, AtraAOX2 does not degrade the carbamate insecticide aldicarb | Amyelois transitella | ? | - |
? | |
1.2.3.1 | nonanal + H2O + O2 | - |
Amyelois transitella | nonanoic acid + H2O2 | - |
? | |
1.2.3.1 | propanal + H2O + O2 | highest AtraAOX2 activity | Amyelois transitella | propanoic acid + H2O2 | - |
? | |
1.2.3.1 | vanillin + H2O + O2 | low AtraAOX2 activity | Amyelois transitella | vanillic acid + H2O2 | - |
? | |
1.2.3.1 | xanthine + H2O + O2 | lowest AtraAOX2 activity | Amyelois transitella | ? + H2O2 | - |
? | |
1.2.3.1 | Z11Z13-16Ald + H2O + O2 | low AtraAOX2 activity | Amyelois transitella | ? + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.3.1 | homodimer | 2 * 140000, SDS-PAGE | Amyelois transitella |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.1 | antennae-specific aldehyde oxidase | - |
Amyelois transitella |
1.2.3.1 | AtraAOX2 | - |
Amyelois transitella |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.3.1 | 20 | 60 | relatively stable from 20 to 60°C but activity drops dramatically at 70°C, AtraAOX2 preheated at 60°C, shows biphasic inactivation, with a rapid decrease from 0 to 10 min, and monotonous decrease thereafter, with an estimated half-life of 33 min | Amyelois transitella |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.3.1 | 8 | - |
enzyme activity tested with three different buffers at pH 8, but no significant difference observed | Amyelois transitella |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.3.1 | 4 | 8 | - |
Amyelois transitella |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.3.1 | physiological function | AtraAOX2 could be involved in detoxifying plant-derived toxic aldehydes and aldehyde-containing pesticides | Amyelois transitella |