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Literature summary extracted from

  • Tran, P.; Sharifi, M.; Poddar, S.; Dent, R.; Niyogi, K.
    Intragenic enhancers and suppressors of phytoene desaturase mutations in Chlamydomonas reinhardtii (2012), PLoS ONE, 7, e42196.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.29 gene pds, DNA and amino acid sequence determination and analysis, sequence comarisons Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.29 E143K naturally occcuring mutation, mutation pds1-1 Chlamydomonas reinhardtii
1.3.99.29 L64F/E143K site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
1.3.99.29 L64P site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
1.3.99.29 L64P/E143K site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
1.3.99.29 L64P/K90M/E143K site-directed mutagenesis, mutation pds1-6, with in-frame deletion of PSY residues 7-14 Chlamydomonas reinhardtii
1.3.99.29 additional information the naturally occuring pds1-1 allele shows a mutant with reduced enzyme activity and growth defects, isolation of pds1-2 as an intragenic enhancer of pds1-1, the pds1-2 and pds1-3 allele products completely lack PDS activity, pds1-3 is a null allele derived from DNA insertional mutagenesis. The null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants. DNA insertional mutagenesis, phenotypes, detailed overview Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.29 norflurazon a bleaching herbicide that specifically inhibits PDS activity and carotenoid biosynthesis Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.29 all-trans-phytofluene + acceptor Chlamydomonas reinhardtii
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.29 all-trans-phytofluene + acceptor Chlamydomonas reinhardtii 4A+
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.29 additional information Chlamydomonas reinhardtii the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene ?
-
 ?
1.3.99.29 additional information Chlamydomonas reinhardtii 4A+ the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.29 Chlamydomonas reinhardtii Q6J213 gene pds
-
1.3.99.29 Chlamydomonas reinhardtii 4A+ Q6J213 gene pds
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.29 all-trans-phytofluene + acceptor
-
 Chlamydomonas reinhardtii all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.29 all-trans-phytofluene + acceptor
-
 Chlamydomonas reinhardtii 4A+ all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.29 additional information the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene Chlamydomonas reinhardtii ?
-
 ?
1.3.99.29 additional information the enzyme also forms 7,9,9'-tricis-neurosporene from 9,9'-dicis-zeta-carotene Chlamydomonas reinhardtii 4A+ ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.29 PDS
-
 Chlamydomonas reinhardtii
1.3.99.29 phytoene desaturase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.29 25
-
 assay at Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
1.3.99.29 malfunction the light-sensitive, phytoene-accumulating mutant, pds1-1, isolated in Chlamydomonas reinhardtii, is leaky for PDS activity. It shows decreased accumulation of downstream colored carotenoids. The pds1-2 and pds1-3 allele products completely lack PDS activity, the null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants Chlamydomonas reinhardtii
1.3.99.29 metabolism the enzyme catalyzes the second step in carotenoid biosynthesis, the conversion of phytoene to zeta-carotene Chlamydomonas reinhardtii
1.3.99.29 additional information intragenic suppressors binding analysis and computational structure prediction of PDS, overview Chlamydomonas reinhardtii