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Literature summary extracted from

  • Shen, S.H.; Wertz, D.L.; Klinman, J.P.
    Implication for functions of the ectopic adipocyte copper amine oxidase (AOC3) from purified enzyme and cell-based kinetic studies (2012), PLoS ONE, 7, e29270.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21 expressed in Drosophila melanogaster Schneider-2 cells and in 3T3-L1 mouse adipocytes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 Semicarbazide
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.031
-
 cysteamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.066
-
 aminoacetone at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.0845
-
 benzylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.099
-
 dopamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.652
-
 methylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 2.05
-
 phenethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 2.65
-
 Propylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 2.83
-
 Butylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 3.42
-
 Isobutylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 4.56
-
 Isoamylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 5.71
-
 amylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 12.8
-
 ethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 16.5
-
 cyclohexanemethylamine at pH 7.4, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 Cu2+ required for activity, the zinc content is 0.4 mol per mole of AOC3 monomer Homo sapiens
1.4.3.21 Zn2+ the zinc content is 0.16 mol per mole of AOC3 monomer Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Homo sapiens Q16853
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21 ammonium sulfate precipitation, Sepharose column chromatography, and Sephacryl S-200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.21 adipocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 aminoacetone + H2O + O2
-
 Homo sapiens methylglyoxal + NH3 + H2O2
-
 ?
1.4.3.21 amylamine + H2O + O2
-
 Homo sapiens pentanal + NH3 + H2O2
-
 ?
1.4.3.21 benzylamine + H2O + O2 best substrate Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 butylamine + H2O + O2
-
 Homo sapiens butanal + NH3 + H2O2
-
 ?
1.4.3.21 cyclohexanemethylamine + H2O + O2
-
 Homo sapiens cyclohexanecarbaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 cysteamine + H2O + O2
-
 Homo sapiens sulfanylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 dopamine + H2O + O2
-
 Homo sapiens ?
-
 ?
1.4.3.21 ethylamine + H2O + O2
-
 Homo sapiens acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 isoamylamine + H2O + O2
-
 Homo sapiens isoamylaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 isobutylamine + H2O + O2
-
 Homo sapiens isobutyraldehyde + NH3 + H2O2
-
 ?
1.4.3.21 methylamine + H2O + O2
-
 Homo sapiens formaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 phenethylamine + H2O + O2
-
 Homo sapiens phenylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 propylamine + H2O + O2
-
 Homo sapiens propanal + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 AOC3
-
 Homo sapiens
1.4.3.21 Copper amine oxidase
-
 Homo sapiens
1.4.3.21 VAP-1
-
 Homo sapiens
1.4.3.21 vascular adhesion protein-1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 0.54
-
 dopamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 1.11
-
 cysteamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 1.12
-
 phenethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 1.46
-
 aminoacetone at pH 7.4, 37°C Homo sapiens
1.4.3.21 3.42
-
 benzylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 4.33
-
 amylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 5.36
-
 cyclohexanemethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 5.6
-
 methylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 5.8
-
 Propylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 6.12
-
 ethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 6.31
-
 Butylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 6.89
-
 Isoamylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 7.4
-
 Isobutylamine at pH 7.4, 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
1.4.3.21 Homo sapiens cells are induced to express the enzyme with 0.6 mM CuSO4 up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.21 0.325
-
 cyclohexanemethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.478
-
 ethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.545
-
 phenethylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 0.757
-
 amylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 1.51
-
 Isoamylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 2.16
-
 Isobutylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 2.19
-
 Propylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 2.23
-
 Butylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 5.44
-
 dopamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 8.58
-
 methylamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 22.3
-
 aminoacetone at pH 7.4, 37°C Homo sapiens
1.4.3.21 35.5
-
 cysteamine at pH 7.4, 37°C Homo sapiens
1.4.3.21 40.5
-
 benzylamine at pH 7.4, 37°C Homo sapiens