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Literature summary extracted from

  • Dobson, R.C.; Giron, I.; Hudson, A.O.
    L,L-diaminopimelate aminotransferase from Chlamydomonas reinhardtii: a target for algaecide development (2011), PLoS ONE, 6, e20439.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.83 S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in complementation experiments Chlamydomonas reinhardtii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.83 PEG 3350, sitting drop method Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.83 0.1
-
 tetrahydrodipicolinate pH 7.6, 30°C Chlamydomonas reinhardtii
2.6.1.83 0.3
-
 LL-diaminopimelate pH 7.6, 30°C Chlamydomonas reinhardtii
2.6.1.83 0.9
-
 L-glutamate pH 7.6, 30°C Chlamydomonas reinhardtii
2.6.1.83 2.2
-
 2-oxoglutarate pH 7.6, 30°C Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.83 50000
-
 2 * 50000, SDS-PAGE, analytical ultracentrifugation Chlamydomonas reinhardtii
2.6.1.83 100200
-
 analytical ultracentrifugation Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.83 Chlamydomonas reinhardtii A8IW39
-
-
2.6.1.83 Chlamydomonas reinhardtii CC-1690 A8IW39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.83 immobilized metal ion affinity chromatography, gel filtration Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.83 0.68
-
 substrates glutamate and tetrahydrodipicolinate, pH 7.6, 30°C Chlamydomonas reinhardtii
2.6.1.83 11.6
-
 substrates LL-diaminopimelate and 2-oxoglutarate, pH 7.6, 30°C Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.83 L-glutamate + tetrahydrodipicolinate + H2O no activity observed with pyruvate, prephenate, oxaloacetate and oxovalerate Chlamydomonas reinhardtii LL-diaminopimelate + 2-oxoglutarate
-
 r
2.6.1.83 L-glutamate + tetrahydrodipicolinate + H2O no activity observed with pyruvate, prephenate, oxaloacetate and oxovalerate Chlamydomonas reinhardtii CC-1690 LL-diaminopimelate + 2-oxoglutarate
-
 r
2.6.1.83 LL-diaminopimelate + 2-oxoglutarate no activity observed with meso-diaminopimelate, L-lysine and L-ornithine Chlamydomonas reinhardtii L-glutamate + tetrahydrodipicolinate + H2O
-
 r
2.6.1.83 LL-diaminopimelate + 2-oxoglutarate no activity observed with meso-diaminopimelate, L-lysine and L-ornithine Chlamydomonas reinhardtii CC-1690 L-glutamate + tetrahydrodipicolinate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.83 homodimer 2 * 50000, SDS-PAGE, analytical ultracentrifugation Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
2.6.1.83 L,L-diaminopimelate aminotransferase
-
 Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.83 1.1
-
 tetrahydrodipicolinate pH 7.6, 30°C Chlamydomonas reinhardtii
2.6.1.83 19
-
 LL-diaminopimelate pH 7.6, 30°C Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.83 pyridoxal 5'-phosphate
-
 Chlamydomonas reinhardtii