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Literature summary extracted from

  • Mendez, V.; Agullo, L.; Gonzalez, M.; Seeger, M.
    The homogentisate and homoprotocatechuate central pathways are involved in 3- and 4-hydroxyphenylacetate degradation by Burkholderia xenovorans LB400 (2011), PLoS ONE, 6, e17583.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.5 genes hmgA1 and hmgA2, the gene cluster hmgABC is located at C1 in strain LB400 genome, quantitative expression analysis Paraburkholderia xenovorans
1.13.11.15 gene hpaD, gene cluster hpaG1G2EDFHI encodes the homoprotocatechuate pathway is located at C2 in strain LB400 genome, quantitative expression analysis Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.5 homogentisate + O2 Paraburkholderia xenovorans
-
 4-maleylacetoacetate
-
 ?
1.13.11.5 additional information Paraburkholderia xenovorans LB400 cells grown with 3-hydroxyphenylacetate degrade homogentisate and show homogentisate 1,2-dioxygenase, EC 1.13.11.5 activity ?
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Paraburkholderia xenovorans
-
 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 additional information Paraburkholderia xenovorans LB400 cells grown with 3-hydroxyphenylacetate degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.5 Paraburkholderia xenovorans
-
 genes hmgA1 and hmgA2
-
1.13.11.15 Paraburkholderia xenovorans
-
 gene hpaD
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.5 culture condition:3-hydroxyphenylacetate-grown cell
-
 Paraburkholderia xenovorans
-
1.13.11.15 culture condition:3-hydroxyphenylacetate-grown cell
-
 Paraburkholderia xenovorans
-
1.13.11.15 culture condition:4-hydroxyphenylacetate-grown cell
-
 Paraburkholderia xenovorans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.5 homogentisate + O2
-
 Paraburkholderia xenovorans 4-maleylacetoacetate
-
 ?
1.13.11.5 additional information LB400 cells grown with 3-hydroxyphenylacetate degrade homogentisate and show homogentisate 1,2-dioxygenase, EC 1.13.11.5 activity Paraburkholderia xenovorans ?
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Paraburkholderia xenovorans 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 additional information LB400 cells grown with 3-hydroxyphenylacetate degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity Paraburkholderia xenovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.5 homogentisate 1,2-dioxygenase
-
 Paraburkholderia xenovorans
1.13.11.15 homoprotocatechuate 2,3-dioxygenase
-
 Paraburkholderia xenovorans
1.13.11.15 HPC 2,3-dioxygenase
-
 Paraburkholderia xenovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.5 30
-
 assay at Paraburkholderia xenovorans
1.13.11.15 30
-
 assay at Paraburkholderia xenovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.5 7
-
 assay at Paraburkholderia xenovorans
1.13.11.15 7
-
 assay at Paraburkholderia xenovorans

Expression

EC Number Organism Comment Expression
1.13.11.5 Paraburkholderia xenovorans induction of fumarylacetoacetate hydrolase HmgB in Burkholderia xenovorans LB400 during growth on hydroxyphenylacetates additional information
1.13.11.5 Paraburkholderia xenovorans LB400 cells grown with 3-hydroxyphenylacetate degrade homogentisate and show homogentisate 1,2-dioxygenase activity. The hmgA1 gene is upregulated by 3-hydroxyphenylacetate in strain LB400. The expression of the hmgA2 gene is induced in 3-hydroxyphenylacetategrown-cells up
1.13.11.15 Paraburkholderia xenovorans induction of fumarylacetoacetate hydrolase HmgB in Burkholderia xenovorans LB400 during growth on hydroxyphenylacetates additional information
1.13.11.15 Paraburkholderia xenovorans LB400 cells grown with 3-hydroxyphenylacetate and 4-hydroxyphenylacetate both show induction of the homoprotocatechuate pathway, they degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity. The hmgA1 gene is upregulated by 3-hydroxyphenylacetate in strain LB400. The expression of the hpaD gene, encoding HPC 2,3-dioxygenase, is induced in 3-hydroxyphenylacetate and 4-hydroxyphenylacetate grown-cells up