EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.3.7 | expressed in Pichia pastoris | Arabidopsis thaliana |
1.2.3.14 | cDNAs of AAO1 and AAO3 expressed in Pichia pastoris to obtain recombinant homodimeric AAO1 and AAO3 proteins with His6-tag | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.7 | diphenylene iodonium | strong inhibitor | Arabidopsis thaliana | |
1.2.3.7 | KCN | complete inhibition at 20 mM after 1 h incubation at room temperature | Arabidopsis thaliana | |
1.2.3.14 | cyanide | the ability of AAO1 and AAO3 to reduce 2,6-dichloroindophenol is abrogated when the enzymes are pre-treated with cyanide, NADH oxidation activity of AAO1 and AAO3 is highly sensitive to cyanide treatment | Arabidopsis thaliana | |
1.2.3.14 | diphenylene iodonium | DPI i.e. diphenylene iodonium, in the presence of DPI aldehyde oxidation activities of AAO1 and AAO3 are strongly reduced to 116%, NADH oxidation activity of AAO1 and AAO3 is highly sensitive to DPI treatment | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.7 | indole-3-acetaldehyde + O2 | Arabidopsis thaliana | highly efficient conversion | indole-3-acetate + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.7 | Arabidopsis thaliana | Q7G193 | - |
- |
1.2.3.14 | Arabidopsis thaliana | Q7G9P4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.3.7 | Ni-NTA agarose column chromatography, and Source 15Q colum chromatography | Arabidopsis thaliana |
1.2.3.14 | by affinity chromatography with nickel-nitrilotriacetic acid-agarose under native conditions, further purification by anion exchange chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.3.7 | seedling | - |
Arabidopsis thaliana | - |
1.2.3.14 | rosette leaf | extracts from drought stressed leaves, AAO3 | Arabidopsis thaliana | - |
1.2.3.14 | seedling | AAO1 | Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.3.7 | 0.093 | - |
using heptaldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.7 | 0.11 | - |
using benzaldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.7 | 0.515 | - |
using abscisic aldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.7 | 0.558 | - |
using indole-3-carbaldehyde as substrate, pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | additional information | - |
in the presence of diphenylene iodonium, aldehyde oxidation activities of AAO1 and AAO3 are strongly reduced to 116% | Arabidopsis thaliana |
1.2.3.14 | 0.093 | - |
heptaldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.11 | - |
benzaldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.146 | - |
benzaldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.204 | - |
indole-3-carbaldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.215 | - |
NADH oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.515 | - |
abscisic aldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.517 | - |
heptaldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.53 | - |
NADH oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.558 | - |
indole-3-carbaldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
1.2.3.14 | 0.635 | - |
abscisic aldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.7 | abscisic aldehyde + oxidized 2,6-dichloroindophenol + H2O | - |
Arabidopsis thaliana | abscisic acid + reduced 2,6-dichloroindophenol | - |
? | |
1.2.3.7 | benzaldehyde + oxidized 2,6-dichloroindophenol + H2O | weak substrate | Arabidopsis thaliana | benzoic acid + reduced 2,6-dichloroindophenol | - |
? | |
1.2.3.7 | heptaldehyde + oxidized 2,6-dichloroindophenol + H2O | weak substrate | Arabidopsis thaliana | heptanoic acid + reduced 2,6-dichloroindophenol | - |
? | |
1.2.3.7 | indole-3-acetaldehyde + O2 | highly efficient conversion | Arabidopsis thaliana | indole-3-acetate + H2O2 | - |
? | |
1.2.3.7 | indole-3-acetaldehyde + oxidized 2,6-dichloroindophenol + H2O | efficient substrate | Arabidopsis thaliana | indole-3-acetate + reduced 2,6-dichloroindophenol | - |
? | |
1.2.3.7 | additional information | in addition to the two-electron reduction of molecular oxygen, isoform AAO1 also catalyzes a one-electron transfer to molecular oxygen, leading to the formation of O2-. However, the production of O2 appears to be less efficient than the production of H2O2 | Arabidopsis thaliana | ? | - |
? | |
1.2.3.14 | abscisic aldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | abscisic acid + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
1.2.3.14 | benzaldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | benzoic acid + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
1.2.3.14 | heptaldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | heptanoic acid + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
1.2.3.14 | indole-3-carbaldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | indole-3-carboxylate + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
1.2.3.14 | additional information | confirmation of superoxide generation by AAO1 and AAO3 by monitoring the reduction of the tetrazolium salt XTT due to O2- | Arabidopsis thaliana | ? | - |
? | |
1.2.3.14 | NADH + O2 | oxidation of NADH by AAO1 and AAO3, no oxidation of NADPH by AAO1 or AAO3 | Arabidopsis thaliana | NAD+ + O2- | for confirmation, O2--dependent reduction of cytochrome c monitored, oxidation of NADH by AAO1 and AAO3 does not result in detectable levels of H2O2 | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.3.7 | homodimer | - |
Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.7 | AAO1 | - |
Arabidopsis thaliana |
1.2.3.7 | AOgamma | - |
Arabidopsis thaliana |
1.2.3.14 | AAO1 | - |
Arabidopsis thaliana |
1.2.3.14 | AAO3 | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.14 | additional information | presence of all prosthetic groups confirmed by UVvis spectroscopy | Arabidopsis thaliana |